biostudies-literatureMeyer LEDanmarks Frie Forskningsfonde202203823https://www.ebi.ac.uk/biostudies/studies/S-EPMC9400879biostudies-literatureUnknown61(31)The applicability of a thermomorphic multiphasic system (TMS) composed of a hydrophobic deep eutectic solvent (DES) and an aqueous potassium phosphate buffer with a lower critical solution temperature (LCST) phase change for homogeneous biocatalysis was investigated. A lidocaine-based DES with the fatty acid oleic acid as a hydrogen-bond donor was studied. Phase diagrams were determined and presented within this study. We tested different additional components to the solvent system and observed a decrease in the cloud point of approximately 0.026 °C per concentration unit. Distribution studies revealed a clear distribution of the protein in the aqueous buffer phase (>95 %), whereas the hydrophobic substrate and educt accumulated (>95 %) in the DES-enriched layer. Finally, a reduction catalAngewandte Chemie (International ed. in English)A Deep Eutectic Solvent Thermomorphic Multiphasic System for Biocatalytic Applications.PMC94008799063 00031BMeyer LEAndersen MBKara SfalseA Deep Eutectic Solvent Thermomorphic Multiphasic System for Biocatalytic Applications.The applicability of a thermomorphic multiphasic system (TMS) composed of a hydrophobic deep eutectic solvent (DES) and an aqueous potassium phosphate buffer with a lower critical solution temperature (LCST) phase change for homogeneous biocatalysis was investigated. A lidocaine-based DES with the fatty acid oleic acid as a hydrogen-bond donor was studied. Phase diagrams were determined and presented within this study. We tested different additional components to the solvent system and observed a decrease in the cloud point of approximately 0.026 °C per concentration unit. Distribution studies revealed a clear distribution of the protein in the aqueous buffer phase (>95 %), whereas the hydrophobic substrate and educt accumulated (>95 %) in the DES-enriched layer. Finally, a reduction catal2022-01-01T00:00:00Z2022 Aug2025-04-04T21:52:15.806Z2025-04-04T21:52:15.806ZS-EPMC94008793558765510.1002/anie.202203823