biostudies-literatureGuo JGuangxi Distinguished Experts Special FundNational Natural Science Foundation of GuangxiNational Natural Science Foundation of ChinaResearch Enhancement Project for Junior Faculty in Higher Education Institutes of GuangxiScience and Technology Planned Project in GuilinSpecial Fund of the Central Government Guiding Local Scientific and Technological Development by Guangxi Science and Technology Departmente13908https://www.ebi.ac.uk/biostudies/studies/S-EPMC9406791biostudies-literatureUnknown10<h4>Background</h4>Lung adenocarcinoma (LUAD) is a major type of lung cancer with poor prognosis and low 5-year survival rate, which urgently needs further investigation in order to elucidate its mechanisms completely and discover novel therapeutic targets. C1orf74 is a novel protein with unknown function either in normal cells or cancer cells. The aim of this study is to investigate the expression and function of C1orf74 in LUAD cells.<h4>Methods</h4>The expression of C1orf74 in LUAD was analyzed using the LUAD datasets from public databases. The prognostic value of C1orf74 in LUAD was analyzed using Kaplan-Meier Plotter. C1orf74 expression in LUAD cell line A549, H1993 and HCC827 was silenced using small interfering RNA, and then the effects of C1orf74 knockdown on proliferation, migratiPeerJC1orf74 positively regulates the EGFR/AKT/mTORC1 signaling in lung adenocarcinoma cells.PMC94067912019B12ZY21195024321601562020GXNSFAA29720920210102-12022KY0480Guo JHuang GLi AGou YGuo RHe QJin JWu YfalseC1orf74 positively regulates the EGFR/AKT/mTORC1 signaling in lung adenocarcinoma cells.<h4>Background</h4>Lung adenocarcinoma (LUAD) is a major type of lung cancer with poor prognosis and low 5-year survival rate, which urgently needs further investigation in order to elucidate its mechanisms completely and discover novel therapeutic targets. C1orf74 is a novel protein with unknown function either in normal cells or cancer cells. The aim of this study is to investigate the expression and function of C1orf74 in LUAD cells.<h4>Methods</h4>The expression of C1orf74 in LUAD was analyzed using the LUAD datasets from public databases. The prognostic value of C1orf74 in LUAD was analyzed using Kaplan-Meier Plotter. C1orf74 expression in LUAD cell line A549, H1993 and HCC827 was silenced using small interfering RNA, and then the effects of C1orf74 knockdown on proliferation, migrati2022-01-01T00:00:00Z20222026-04-08T12:01:58.437Z2025-02-19T04:38:12.87ZS-EPMC94067913603296010.7717/peerj.13908