biostudies-literatureMa QChina Agriculture Research SystemShanghai Natural Science FoundationNational Natural Science Foundation of ChinaNational Key Research and Development Project of China9231https://www.ebi.ac.uk/biostudies/studies/S-EPMC9409078biostudies-literatureUnknown23(16)Cassava storage roots are an important source of food, feed, and material for starch-based industries in many countries. After harvest, rapid post-harvest physiological deterioration (PPD) reduces their palatability and marketability. During the PPD process, vascular streaking occurs through over-accumulation of coumarins, the biosynthesis of which involves the key enzyme <i>p</i>-coumaroyl shikimate/quinate 3'-hydroxylase (C3'H). Repression of <i>MeC3'H</i> expression by RNA interference in transgenic cassava plants caused a significant delay in PPD by decreasing scopoletin and scopolin accumulation in field-harvested storage roots. This study demonstrates that MeC3'H is the key enzyme participating in coumarin biosynthesis during PPD and shows that <i>MeC3'H</i> is a useful target gene fInternational journal of molecular sciencesKnockdown of <i>p</i>-Coumaroyl Shikimate/Quinate 3'-Hydroxylase Delays the Occurrence of Post-Harvest Physiological Deterioration in Cassava Storage Roots.PMC9409078No. 31801417No. 2019YFD1001100No. CARS-11No. 17ZR1435200Ma QLu XFeng YXu JWu XZhang PfalseKnockdown of <i>p</i>-Coumaroyl Shikimate/Quinate 3'-Hydroxylase Delays the Occurrence of Post-Harvest Physiological Deterioration in Cassava Storage Roots.Cassava storage roots are an important source of food, feed, and material for starch-based industries in many countries. After harvest, rapid post-harvest physiological deterioration (PPD) reduces their palatability and marketability. During the PPD process, vascular streaking occurs through over-accumulation of coumarins, the biosynthesis of which involves the key enzyme <i>p</i>-coumaroyl shikimate/quinate 3'-hydroxylase (C3'H). Repression of <i>MeC3'H</i> expression by RNA interference in transgenic cassava plants caused a significant delay in PPD by decreasing scopoletin and scopolin accumulation in field-harvested storage roots. This study demonstrates that MeC3'H is the key enzyme participating in coumarin biosynthesis during PPD and shows that <i>MeC3'H</i> is a useful target gene f2022-01-01T00:00:00Z2022 Aug2025-05-29T16:11:46.738Z2025-04-04T09:32:22.493ZS-EPMC94090783601249610.3390/ijms23169231