biostudies-literatureHu HNCI NIH HHSNIH1298-1315https://www.ebi.ac.uk/biostudies/studies/S-EPMC9414288biostudies-literatureUnknown9(11)Substantial progress has been made in understanding how tumors escape immune surveillance. However, few measures to counteract tumor immune evasion have been developed. Suppression of tumor antigen expression is a common adaptive mechanism that cancers use to evade detection and destruction by the immune system. Epigenetic modifications play a critical role in various aspects of immune invasion, including the regulation of tumor antigen expression. To identify epigenetic regulators of tumor antigen expression, we established a transplantable syngeneic tumor model of immune escape with silenced antigen expression and used this system as a platform for a CRISPR-Cas9 suppressor screen for genes encoding epigenetic modifiers. We found that disruption of the genes encoding either of the chromatCancer immunology researchTargeting the Atf7ip-Setdb1 Complex Augments Antitumor Immunity by Boosting Tumor Immunogenicity.PMC9414288RO1CA219670RO1CA248896R01 CA215471R01 CA248896R01 CA219670RO1CA215471Ueberheide BSucker AZeck BZhang HSchadendorf DDeng JOsman IWong KKChiriboga LADowling CMCho HChen ZHuang HYBadri SZappile PTang SDolgalev IChen TVelcheti VRanieri MJi HLi FLabbe KJin YPoirier JTTsirigos AJour GKaratza ASchalper KALopez De Rodas Gregorio MHu HHan HKhodadadi-Jamayran AfalseTargeting the Atf7ip-Setdb1 Complex Augments Antitumor Immunity by Boosting Tumor Immunogenicity.Substantial progress has been made in understanding how tumors escape immune surveillance. However, few measures to counteract tumor immune evasion have been developed. Suppression of tumor antigen expression is a common adaptive mechanism that cancers use to evade detection and destruction by the immune system. Epigenetic modifications play a critical role in various aspects of immune invasion, including the regulation of tumor antigen expression. To identify epigenetic regulators of tumor antigen expression, we established a transplantable syngeneic tumor model of immune escape with silenced antigen expression and used this system as a platform for a CRISPR-Cas9 suppressor screen for genes encoding epigenetic modifiers. We found that disruption of the genes encoding either of the chromat2021-01-01T00:00:00Z2021 Nov2025-04-04T13:08:56.235Z2025-04-04T13:08:56.235ZS-EPMC94142883446228410.1158/2326-6066.CIR-21-0543