biostudies-literatureSong YFNatural Science Special Project in Guizhou UniversityGuizhou Province Science and Technology Innovation Talent Team ProjectNational Natural Science Foundation of ChinaHebei Provincial Key R&amp;amp;D ProgramHigh-level Talent Innovation and Entrepreneurship Funding Project in Guizhou ProvinceGrowth Project of Youth Talent in Ordinary Universities in Guizhou Province571https://www.ebi.ac.uk/biostudies/studies/S-EPMC9415403biostudies-literatureUnknown14(8)<i>Pyemotes zhonghuajia</i> Yu, Zhang & He (Prostigmata: Pyemotidae), discovered in China, has been demonstrated as a high-efficient natural enemy in controlling many agricultural and forestry pests. This mite injects toxins into the host (eggs, larvae, pupae, and adults), resulting in its paralyzation and then gets nourishment for reproductive development. These toxins have been approved to be mammal-safe, which have the potential to be used as biocontrol pesticides. Toxin proteins have been identified from many insects, especially those from the orders Scorpions and Araneae, some of which are now widely used as efficient biocontrol pesticides. However, toxin proteins in mites are not yet understood. In this study, we assembled the genome of <i>P. zhonghuajia</i> using PacBio technology aToxinsA Long-Read Genome Assembly of a Native Mite in China <i>Pyemotes zhonghuajia</i> Yu, Zhang & He (Prostigmata: Pyemotidae) Reveals Gene Expansion in Toxin-Related Gene Families.PMC9415403[2020]-02[2021]07932060637[2021]01[2021]0043177069420326517DYu LCSong YFLi LTYang MFYe SWu CLiu JFYan BfalseA Long-Read Genome Assembly of a Native Mite in China <i>Pyemotes zhonghuajia</i> Yu, Zhang & He (Prostigmata: Pyemotidae) Reveals Gene Expansion in Toxin-Related Gene Families.<i>Pyemotes zhonghuajia</i> Yu, Zhang & He (Prostigmata: Pyemotidae), discovered in China, has been demonstrated as a high-efficient natural enemy in controlling many agricultural and forestry pests. This mite injects toxins into the host (eggs, larvae, pupae, and adults), resulting in its paralyzation and then gets nourishment for reproductive development. These toxins have been approved to be mammal-safe, which have the potential to be used as biocontrol pesticides. Toxin proteins have been identified from many insects, especially those from the orders Scorpions and Araneae, some of which are now widely used as efficient biocontrol pesticides. However, toxin proteins in mites are not yet understood. In this study, we assembled the genome of <i>P. zhonghuajia</i> using PacBio technology a2022-01-01T00:00:00Z2022 Aug2025-08-30T03:12:50.728Z2025-04-07T10:35:04.543ZS-EPMC94154033600623310.3390/toxins14080571