{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["5(1)"],"submitter":["Tang WC"],"pubmed_abstract":["Lattice lightsheet microscopy (LLSM) featuring three-dimensional recording is improved to manipulate cellular behavior with subcellular resolution through optogenetic activation (optoLLSM). A position-controllable Bessel beam as a stimulation source is integrated into the LLSM to achieve spatiotemporal photoactivation by changing the spatial light modulator (SLM) patterns. Unlike the point-scanning in a confocal microscope, the lattice beams are capable of wide-field optical sectioning for optogenetic activation along the Bessel beam path.We show that the energy power required for optogenetic activations is lower than 1 nW (or 24 mWcm<sup>-2</sup>) for time-lapses of CRY2olig clustering proteins, and membrane ruffling can be induced at different locations within a cell with subcellular res"],"journal":["Communications biology"],"pagination":["879"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC9418249"],"repository":["biostudies-literature"],"pubmed_title":["Optogenetic manipulation of cell migration with high spatiotemporal resolution using lattice lightsheet microscopy."],"pmcid":["PMC9418249"],"pubmed_authors":["Tu CH","Lin YC","Chen P","Chang SW","Yeh CH","Gao L","Tang WC","Lu CH","Lin YL","Chen BC","Hsu TL","Liu YT"],"additional_accession":[]},"is_claimable":false,"name":"Optogenetic manipulation of cell migration with high spatiotemporal resolution using lattice lightsheet microscopy.","description":"Lattice lightsheet microscopy (LLSM) featuring three-dimensional recording is improved to manipulate cellular behavior with subcellular resolution through optogenetic activation (optoLLSM). A position-controllable Bessel beam as a stimulation source is integrated into the LLSM to achieve spatiotemporal photoactivation by changing the spatial light modulator (SLM) patterns. Unlike the point-scanning in a confocal microscope, the lattice beams are capable of wide-field optical sectioning for optogenetic activation along the Bessel beam path.We show that the energy power required for optogenetic activations is lower than 1 nW (or 24 mWcm<sup>-2</sup>) for time-lapses of CRY2olig clustering proteins, and membrane ruffling can be induced at different locations within a cell with subcellular res","dates":{"release":"2022-01-01T00:00:00Z","publication":"2022 Aug","modification":"2025-04-18T22:52:24.84Z","creation":"2025-04-07T10:35:27.083Z"},"accession":"S-EPMC9418249","cross_references":{"pubmed":["36028551"],"doi":["10.1038/s42003-022-03835-6"]}}