biostudies-literatureUnknown5(1)Tang WCLattice lightsheet microscopy (LLSM) featuring three-dimensional recording is improved to manipulate cellular behavior with subcellular resolution through optogenetic activation (optoLLSM). A position-controllable Bessel beam as a stimulation source is integrated into the LLSM to achieve spatiotemporal photoactivation by changing the spatial light modulator (SLM) patterns. Unlike the point-scanning in a confocal microscope, the lattice beams are capable of wide-field optical sectioning for optogenetic activation along the Bessel beam path.We show that the energy power required for optogenetic activations is lower than 1 nW (or 24 mWcm^-2) for time-lapses of CRY2olig clustering proteins, and membrane ruffling can be induced at different locations within a cell with subcellular resCommunications biology879https://www.ebi.ac.uk/biostudies/studies/S-EPMC9418249biostudies-literatureOptogenetic manipulation of cell migration with high spatiotemporal resolution using lattice lightsheet microscopy.PMC9418249Tu CHLin YCChen PChang SWYeh CHGao LTang WCLu CHLin YLChen BCHsu TLLiu YTfalseOptogenetic manipulation of cell migration with high spatiotemporal resolution using lattice lightsheet microscopy.Lattice lightsheet microscopy (LLSM) featuring three-dimensional recording is improved to manipulate cellular behavior with subcellular resolution through optogenetic activation (optoLLSM). A position-controllable Bessel beam as a stimulation source is integrated into the LLSM to achieve spatiotemporal photoactivation by changing the spatial light modulator (SLM) patterns. Unlike the point-scanning in a confocal microscope, the lattice beams are capable of wide-field optical sectioning for optogenetic activation along the Bessel beam path.We show that the energy power required for optogenetic activations is lower than 1 nW (or 24 mWcm^-2) for time-lapses of CRY2olig clustering proteins, and membrane ruffling can be induced at different locations within a cell with subcellular res2022-01-01T00:00:00Z2022 Aug2025-04-18T22:52:24.84Z2025-04-07T10:35:27.083ZS-EPMC94182493602855110.1038/s42003-022-03835-6