biostudies-literatureBuchroithner BAustrian Science Fund FWF2422-2428https://www.ebi.ac.uk/biostudies/studies/S-EPMC9418552biostudies-literatureUnknown2(6)The fabrication of two- and three-dimensional scaffolds mimicking the extracellular matrix and providing cell stimulation is of high importance in biology and material science. We show two new, biocompatible polymers, which can be 3D structured <i>via</i> multiphoton lithography, and determine their mechanical properties. Atomic force microscopy analysis of structures with sub-micron feature sizes reveals Young's modulus values in the 100 MPa range. Assessment of biocompatibility of the new resins was done by cultivating human umbilical vein endothelial cells on two-dimensionally structured substrates for four days. The cell density and presence of apoptotic cells has been quantified.Nanoscale advances3D multiphoton lithography using biocompatible polymers with specific mechanical properties.PMC9418552P 31827-B21Oh YJGriesser TKlar TAJacak JMayr SHinterdorfer PBuchegger BBuchroithner BHartmann DSivun DKarner Afalse3D multiphoton lithography using biocompatible polymers with specific mechanical properties.The fabrication of two- and three-dimensional scaffolds mimicking the extracellular matrix and providing cell stimulation is of high importance in biology and material science. We show two new, biocompatible polymers, which can be 3D structured <i>via</i> multiphoton lithography, and determine their mechanical properties. Atomic force microscopy analysis of structures with sub-micron feature sizes reveals Young's modulus values in the 100 MPa range. Assessment of biocompatibility of the new resins was done by cultivating human umbilical vein endothelial cells on two-dimensionally structured substrates for four days. The cell density and presence of apoptotic cells has been quantified.2020-01-01T00:00:00Z2020 Jun2025-04-04T19:27:56.692Z2025-02-19T01:38:09.784ZS-EPMC94185523613339210.1039/d0na00154f