{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["17(8)"],"submitter":["Marashi H"],"pubmed_abstract":["CCL21 has an essential role in anti-tumor immune activity. Epitopes of IL1β have adjuvant activity without causing inflammatory responses. CCR7 and its ligands play a vital role in the immune balance; specifically, in transport of T lymphocytes and antigen-presenting cells such as dendritic cells to the lymph nodes. This study aimed to produce epitopes of CCL21 and IL1β as a recombinant protein and characterize its in vitro anti-tumor and immunogenic activity. A codon-optimized ccl21/IL1β gene was designed and synthesized from human genes. Stability and binding affinity of CCL21/IL1β protein and CCR7 receptor were examined through in silico analyses. The construct was introduced into N. tabacum to produce this recombinant protein and the structure and function of CCL21/IL1β were examined. "],"journal":["PloS one"],"pagination":["e0261101"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC9423642"],"repository":["biostudies-literature"],"pubmed_title":["In silico analysis and in planta production of recombinant ccl21/IL1β protein and characterization of its in vitro anti-tumor and immunogenic activity."],"pmcid":["PMC9423642"],"pubmed_authors":["Marashi H","Chaboksavar M","Beihaghi M","Khaksar S","Abiri A","Tehrani H"],"additional_accession":[]},"is_claimable":false,"name":"In silico analysis and in planta production of recombinant ccl21/IL1β protein and characterization of its in vitro anti-tumor and immunogenic activity.","description":"CCL21 has an essential role in anti-tumor immune activity. Epitopes of IL1β have adjuvant activity without causing inflammatory responses. CCR7 and its ligands play a vital role in the immune balance; specifically, in transport of T lymphocytes and antigen-presenting cells such as dendritic cells to the lymph nodes. This study aimed to produce epitopes of CCL21 and IL1β as a recombinant protein and characterize its in vitro anti-tumor and immunogenic activity. A codon-optimized ccl21/IL1β gene was designed and synthesized from human genes. Stability and binding affinity of CCL21/IL1β protein and CCR7 receptor were examined through in silico analyses. The construct was introduced into N. tabacum to produce this recombinant protein and the structure and function of CCL21/IL1β were examined. ","dates":{"release":"2022-01-01T00:00:00Z","publication":"2022","modification":"2026-05-28T04:08:07.176Z","creation":"2024-11-08T14:33:57.281Z"},"accession":"S-EPMC9423642","cross_references":{"pubmed":["36037155"],"doi":["10.1371/journal.pone.0261101"]}}