biostudies-literatureZhang YNature Science Foundation of Guangdong Provincethe Undergraduate Research Project of South China Agricultural Universitythe Key Research and Development Program of Guangdong Province1446-1454https://www.ebi.ac.uk/biostudies/studies/S-EPMC9423819biostudies-literatureUnknown13(1)Rabies is an important zoonotic disease caused by the rabies virus (RABV). Currently, no effective treatment is available for this condition. The prevention and control of rabies mainly depend on effective vaccination. Therefore, it is crucial to enhance the immune responses induced by the rabies vaccine. Virus neutralizing antibodies (VNA) induced by rabies vaccines are important for the clearance of RABV. Interleukin-25 (IL-25) has been demonstrated to activate T helper type 2 cells that contribute to humoral immune responses. The IL-25 gene was inserted into the genome of RABV, and the immunogenicity of recombinant RABV with IL-25 gene was investigated to develop more efficient rabies vaccines. Here, we found that the expression of IL-25 did not affect RABV production <i>in vitro</i> anVirulenceInterleukin-25 enhances humoral immune responses caused by the rabies virus.PMC94238192020105640412018A0303131632022A15150125302019B020218004Luo JZhang YLiao XGuo XLiu QZhang MLuo YYu YfalseInterleukin-25 enhances humoral immune responses caused by the rabies virus.Rabies is an important zoonotic disease caused by the rabies virus (RABV). Currently, no effective treatment is available for this condition. The prevention and control of rabies mainly depend on effective vaccination. Therefore, it is crucial to enhance the immune responses induced by the rabies vaccine. Virus neutralizing antibodies (VNA) induced by rabies vaccines are important for the clearance of RABV. Interleukin-25 (IL-25) has been demonstrated to activate T helper type 2 cells that contribute to humoral immune responses. The IL-25 gene was inserted into the genome of RABV, and the immunogenicity of recombinant RABV with IL-25 gene was investigated to develop more efficient rabies vaccines. Here, we found that the expression of IL-25 did not affect RABV production <i>in vitro</i> an2022-01-01T00:00:00Z2022 Dec2025-04-18T18:41:22.905Z2025-02-19T03:28:58.97ZS-EPMC94238193599977610.1080/21505594.2022.2116146