biostudies-literatureKoppenhofer SDeutsche Forschungsgemeinschafte0026422https://www.ebi.ac.uk/biostudies/studies/S-EPMC9426580biostudies-literatureUnknown7(4)The model organism Dinoroseobacter shibae and many other marine <i>Rhodobacterales</i> (<i>Roseobacteraceae</i>, <i>Alphaproteobacteria</i>) are characterized by a multipartite genome organization. Here, we show that the original isolate (Dshi-6) contained six extrachromosomal replicons (ECRs), whereas the strain deposited at the DSMZ (Dshi-5) lacked a 102-kb plasmid. To determine the role of the sixth plasmid, we investigated the genomic and physiological differences between the two strains. Therefore, both genomes were (re)sequenced, and gene expression, growth, and substrate utilization were examined. For comparison, we included additional plasmid-cured strains in the analysis. In the Dshi-6 population, the conjugative 102-kb RepABC-9 plasmid was present in only about 50% of the cells, mSystemsThe Sixth Element: a 102-kb RepABC Plasmid of Xenologous Origin Modulates Chromosomal Gene Expression in Dinoroseobacter shibae.PMC942658034509606 - TRR 51Brinkmann HRingel VTomasch JSproer CJarek MWagner-Dobler IPetersen JKoppenhofer SWang HBirmes LPradella SfalseThe Sixth Element: a 102-kb RepABC Plasmid of Xenologous Origin Modulates Chromosomal Gene Expression in Dinoroseobacter shibae.The model organism Dinoroseobacter shibae and many other marine <i>Rhodobacterales</i> (<i>Roseobacteraceae</i>, <i>Alphaproteobacteria</i>) are characterized by a multipartite genome organization. Here, we show that the original isolate (Dshi-6) contained six extrachromosomal replicons (ECRs), whereas the strain deposited at the DSMZ (Dshi-5) lacked a 102-kb plasmid. To determine the role of the sixth plasmid, we investigated the genomic and physiological differences between the two strains. Therefore, both genomes were (re)sequenced, and gene expression, growth, and substrate utilization were examined. For comparison, we included additional plasmid-cured strains in the analysis. In the Dshi-6 population, the conjugative 102-kb RepABC-9 plasmid was present in only about 50% of the cells, 2022-01-01T00:00:00Z2022 Aug2026-06-13T06:03:57.208Z2025-02-19T03:28:48.434ZS-EPMC94265803592054810.1128/msystems.00264-22