biostudies-literatureNowak JKEuropean Union’s Seventh Framework ProgrammeEuropean Union's Seventh Framework ProgrammeMedical Research CouncilPolish National Science CentreCrohn's and Colitis UK1255-1268https://www.ebi.ac.uk/biostudies/studies/S-EPMC9426667biostudies-literatureUnknown16(8)<h4>Aim</h4>To assess the pathobiological and translational importance of whole-blood transcriptomic analysis in inflammatory bowel disease [IBD].<h4>Methods</h4>We analysed whole-blood expression profiles from paired-end sequencing in a discovery cohort of 590 Europeans recruited across six countries in the IBD Character initiative (newly diagnosed patients with Crohn's disease [CD; n = 156], ulcerative colitis [UC; n = 167], and controls [n = 267]), exploring differential expression [DESeq2], co-expression networks [WGCNA], and transcription factor involvement [EPEE, ChEA, DoRothEA]. Findings were validated by analysis of an independent replication cohort [99 CD, 100 UC, 95 controls]. In the discovery cohort, we also defined baseline expression correlates of future treatment escalation uJournal of Crohn's & colitisCharacterisation of the Circulating Transcriptomic Landscape in Inflammatory Bowel Disease Provides Evidence for Dysregulation of Multiple Transcription Factors Including NFE2, SPI1, CEBPB, and IRF2.PMC94266672858546MR/S034919/1G07018982017/25/B/NZ5/02783M2018-2 HoKalla RA Dahl FNimmo ERNowak JKCasen CTorkvist LHjelm FShand AGSatsangi JFrengen ABSoderholm JDOstrowski JGut IGBonfiglio FLindstrom JCCarstens AOlbjorn CEkeland GSLindahl TDetlie TEHjortswang HNoble CLBergemalm DVentham NTCiemniejewska EEkman DPoncelet DWalkowiak JHo GTSolvernes JIBD Character ConsortiumVatn SAndersson EGullberg MSundell MNordberg NOcklind AArnott IDHalfvarson JGomollon FSchoemans RD'Amato MPierik MGut MPettersson ESabatel CWilson DCJahnsen JMerkel ABoyapati RKKennedy NAHeath SCLees CWJonkers DLindqvist MVatn MHMoen AEFNilsen HBayes MKeita AVVeillard ACYou PDrummond HEAdams ATModig EO'Leary KRTannæs TMRepsilber DRicanek PfalseCharacterisation of the Circulating Transcriptomic Landscape in Inflammatory Bowel Disease Provides Evidence for Dysregulation of Multiple Transcription Factors Including NFE2, SPI1, CEBPB, and IRF2.<h4>Aim</h4>To assess the pathobiological and translational importance of whole-blood transcriptomic analysis in inflammatory bowel disease [IBD].<h4>Methods</h4>We analysed whole-blood expression profiles from paired-end sequencing in a discovery cohort of 590 Europeans recruited across six countries in the IBD Character initiative (newly diagnosed patients with Crohn's disease [CD; n = 156], ulcerative colitis [UC; n = 167], and controls [n = 267]), exploring differential expression [DESeq2], co-expression networks [WGCNA], and transcription factor involvement [EPEE, ChEA, DoRothEA]. Findings were validated by analysis of an independent replication cohort [99 CD, 100 UC, 95 controls]. In the discovery cohort, we also defined baseline expression correlates of future treatment escalation u2022-01-01T00:00:00Z2022 Aug2026-05-31T17:00:15.598Z2024-11-07T03:06:56.009ZS-EPMC94266673521236610.1093/ecco-jcc/jjac033