{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Ma X"],"funding":["National Natural Science Foundation Youth Fund"],"pagination":["1755416"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC9427322"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["2022"],"pubmed_abstract":["<h4>Objectives</h4>Following cerebral ischemia, microRNA- (miR-) 29b in circulating blood is downregulated. This study investigates the underlying mechanism and implications of miR-29b in leukocyte induction.<h4>Methods</h4>miR-29b from stroke patients and rats with middle cerebral artery occlusion (MCAO) were assessed using real-time polymerase chain reaction (PCR). miR-29b agomir was used to increase miR-29b expression in leukocytes via intravenous injection. C1q and tumor necrosis factor (C1QTNF) 6, interleukin- (IL-) 1<i>β</i>, zonula occludens- (ZO-) 1, occludin, and ischemic outcomes were assessed in MCAO rats. Additionally, hCMEC/D3 cells were subjected to oxygen-glucose deprivation (OGD) and cocultured with HL-60 cells.<h4>Results</h4>miR-29b levels in neutrophils were found to be "],"journal":["Mediators of inflammation"],"pubmed_title":["MicroRNA-29b Suppresses Inflammation and Protects Blood-Brain Barrier Integrity in Ischemic Stroke."],"pmcid":["PMC9427322"],"funding_grant_id":["81901194"],"pubmed_authors":["Li G","Yun HJ","Ding Y","Guo Y","Ma X","Elkin K"],"additional_accession":[]},"is_claimable":false,"name":"MicroRNA-29b Suppresses Inflammation and Protects Blood-Brain Barrier Integrity in Ischemic Stroke.","description":"<h4>Objectives</h4>Following cerebral ischemia, microRNA- (miR-) 29b in circulating blood is downregulated. This study investigates the underlying mechanism and implications of miR-29b in leukocyte induction.<h4>Methods</h4>miR-29b from stroke patients and rats with middle cerebral artery occlusion (MCAO) were assessed using real-time polymerase chain reaction (PCR). miR-29b agomir was used to increase miR-29b expression in leukocytes via intravenous injection. C1q and tumor necrosis factor (C1QTNF) 6, interleukin- (IL-) 1<i>β</i>, zonula occludens- (ZO-) 1, occludin, and ischemic outcomes were assessed in MCAO rats. Additionally, hCMEC/D3 cells were subjected to oxygen-glucose deprivation (OGD) and cocultured with HL-60 cells.<h4>Results</h4>miR-29b levels in neutrophils were found to be ","dates":{"release":"2022-01-01T00:00:00Z","publication":"2022","modification":"2025-04-05T00:42:31.878Z","creation":"2025-02-19T03:28:51.777Z"},"accession":"S-EPMC9427322","cross_references":{"pubmed":["36052307"],"doi":["10.1155/2022/1755416"]}}