{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["24(7)"],"submitter":["Heidari Moghadam A"],"pubmed_abstract":["<h4>Objective</h4>The main objective of this study is to determine the myogenic effects of skeletal muscle extracellular matrix, vascular endothelial growth factor and human umbilical vein endothelial cells on adipose-derived stem cells to achieve a 3-dimensional engineered vascular-muscle structure.<h4>Materials and methods</h4>The present experimental research was designed based on two main groups, i.e. monoculture of adipose tissue-derived stem cells (ADSCs) and co-culture of ADSCs and human umbilical vein endothelial cells (HUVECs) in a ratio of 1:1. Skeletal muscle tissue was isolated, decellularized and its surface was electrospun using polycaprolactone/gelatin parallel nanofibers and then matrix topography was evaluated through H and E, trichrome staining and SEM. The expression of "],"journal":["Cell journal"],"pagination":["380-390"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC9428474"],"repository":["biostudies-literature"],"pubmed_title":["Redesigning of 3-Dimensional Vascular-Muscle Structure Using ADSCs/HUVECs Co-Culture and VEGF on Engineered Skeletal Muscle ECM."],"pmcid":["PMC9428474"],"pubmed_authors":["Bayati V","Orazizadeh M","Rashno M","Heidari Moghadam A"],"additional_accession":[]},"is_claimable":false,"name":"Redesigning of 3-Dimensional Vascular-Muscle Structure Using ADSCs/HUVECs Co-Culture and VEGF on Engineered Skeletal Muscle ECM.","description":"<h4>Objective</h4>The main objective of this study is to determine the myogenic effects of skeletal muscle extracellular matrix, vascular endothelial growth factor and human umbilical vein endothelial cells on adipose-derived stem cells to achieve a 3-dimensional engineered vascular-muscle structure.<h4>Materials and methods</h4>The present experimental research was designed based on two main groups, i.e. monoculture of adipose tissue-derived stem cells (ADSCs) and co-culture of ADSCs and human umbilical vein endothelial cells (HUVECs) in a ratio of 1:1. Skeletal muscle tissue was isolated, decellularized and its surface was electrospun using polycaprolactone/gelatin parallel nanofibers and then matrix topography was evaluated through H and E, trichrome staining and SEM. The expression of ","dates":{"release":"2022-01-01T00:00:00Z","publication":"2022 Jul","modification":"2025-04-27T01:52:57.26Z","creation":"2024-10-17T23:33:13.59Z"},"accession":"S-EPMC9428474","cross_references":{"pubmed":["36043406"],"doi":["10.22074/cellj.2022.8098"]}}