{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["10(4)"],"submitter":["Hou N"],"funding":["Westlake Education Foundation","Tencent"],"pubmed_abstract":["The 3C-like protease (3CLpro) of SARS-CoV-2 is an attractive drug target for developing antivirals against SARS-CoV-2. A few small molecule inhibitors of 3CLpro are in clinical trials for COVID-19 treatments, and more inhibitors are under development. One limiting factor for 3CLpro inhibitors development is that the cellular activities of such inhibitors should be evaluated in Biosafety Level 3 (BSL-3) laboratories. Here, we design DNA-coded biosensors that can be used in BSL-2 laboratories to set up cell-based assays for 3CLpro inhibitor discovery. The biosensors were constructed by linking a green fluorescent protein (GFP2) to the N-terminus and a Renilla luciferase (RLuc8) to the C-terminus of SARS-CoV-2 3CLpro, with the linkers derived from the cleavage sequences of 3CLpro. After overe"],"journal":["Microbiology spectrum"],"pagination":["e0255921"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC9430692"],"repository":["biostudies-literature"],"pubmed_title":["BRET-Based Self-Cleaving Biosensors for SARS-CoV-2 3CLpro Inhibitor Discovery."],"pmcid":["PMC9430692"],"pubmed_authors":["Zhu Y","Hou N","Peng C","Zhang L","Hu Q"],"additional_accession":[]},"is_claimable":false,"name":"BRET-Based Self-Cleaving Biosensors for SARS-CoV-2 3CLpro Inhibitor Discovery.","description":"The 3C-like protease (3CLpro) of SARS-CoV-2 is an attractive drug target for developing antivirals against SARS-CoV-2. A few small molecule inhibitors of 3CLpro are in clinical trials for COVID-19 treatments, and more inhibitors are under development. One limiting factor for 3CLpro inhibitors development is that the cellular activities of such inhibitors should be evaluated in Biosafety Level 3 (BSL-3) laboratories. Here, we design DNA-coded biosensors that can be used in BSL-2 laboratories to set up cell-based assays for 3CLpro inhibitor discovery. The biosensors were constructed by linking a green fluorescent protein (GFP2) to the N-terminus and a Renilla luciferase (RLuc8) to the C-terminus of SARS-CoV-2 3CLpro, with the linkers derived from the cleavage sequences of 3CLpro. After overe","dates":{"release":"2022-01-01T00:00:00Z","publication":"2022 Aug","modification":"2026-06-15T06:04:59.965Z","creation":"2025-02-19T02:49:17.682Z"},"accession":"S-EPMC9430692","cross_references":{"pubmed":["35758897"],"doi":["10.1128/spectrum.02559-21"]}}