biostudies-literatureUnknown12(1)Kowatschew DUniversität zu KölnThe vertebrate sense of smell employs four main receptor families for detection of odors, among them the V1R/ORA family, which is unusually small and highly conserved in teleost fish. Zebrafish possess just seven ORA receptors, enabling a comprehensive analysis of the expression patterns of the entire family. The olfactory organ of zebrafish is representative for teleosts, cup-shaped, with lamella covered with sensory epithelium protruding into the cup from a median raphe. We have performed quantitative in situ hybridization on complete series of horizontal cryostat sections of adult zebrafish olfactory organ, and have analysed the location of ora-expressing cells in three dimensions, radial diameter, laminar height, and height-within-the-organ. We report broadly overlapping, but distinctlScientific reports14816https://www.ebi.ac.uk/biostudies/studies/S-EPMC9433392biostudies-literatureSpatial organization of olfactory receptor gene choice in the complete V1R-related ORA family of zebrafish.PMC9433392Bozorg Nia SWeth FKorsching SIUstinova JHassan SKowatschew DfalseSpatial organization of olfactory receptor gene choice in the complete V1R-related ORA family of zebrafish.The vertebrate sense of smell employs four main receptor families for detection of odors, among them the V1R/ORA family, which is unusually small and highly conserved in teleost fish. Zebrafish possess just seven ORA receptors, enabling a comprehensive analysis of the expression patterns of the entire family. The olfactory organ of zebrafish is representative for teleosts, cup-shaped, with lamella covered with sensory epithelium protruding into the cup from a median raphe. We have performed quantitative in situ hybridization on complete series of horizontal cryostat sections of adult zebrafish olfactory organ, and have analysed the location of ora-expressing cells in three dimensions, radial diameter, laminar height, and height-within-the-organ. We report broadly overlapping, but distinctl2022-01-01T00:00:00Z2022 Aug2025-04-19T10:38:04.023Z2025-04-19T10:38:04.023ZS-EPMC94333923604521810.1038/s41598-022-17900-x