{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Kai F"],"funding":["Breast Cancer Research Foundation","Pew Charitable Trusts","Banting Research Foundation","Medical Research Council","NCI NIH HHS","National Institutes of Health","National Institute of General Medical Sciences","American Association for Cancer Research","NIGMS NIH HHS","Canadian Institutes of Health Research","NIH HHS","National Science Foundation"],"pagination":["e109205"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC9434103"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["41(17)"],"pubmed_abstract":["Patient-derived organoids and cellular spheroids recapitulate tissue physiology with remarkable fidelity. We investigated how engagement with a reconstituted basement membrane in three dimensions (3D) supports the polarized, stress resilient tissue phenotype of mammary epithelial spheroids. Cells interacting with reconstituted basement membrane in 3D had reduced levels of total and actin-associated filamin and decreased cortical actin tension that increased plasma membrane protrusions to promote negative plasma membrane curvature and plasma membrane protein associations linked to protein secretion. By contrast, cells engaging a reconstituted basement membrane in 2D had high cortical actin tension that forced filamin unfolding and endoplasmic reticulum (ER) associations. Enhanced filamin-ER"],"journal":["The EMBO journal"],"pubmed_title":["ECM dimensionality tunes actin tension to modulate endoplasmic reticulum function and spheroid phenotypes of mammary epithelial cells."],"pmcid":["PMC9434103"],"funding_grant_id":["R01CA222508‐01","R35 GM136420","P01GM121203","A132292","864K625","R35 GM141832","CA227550","U01 CA202241","U01CA250044‐01A1","GM141832","Postdoctoral Fellowship","U01 CA202123","U01CA202241","NCI1U01CA202123","U01 CA250044","Basic Cancer Research Fellowship","R35CA242447‐01A1","R01 CA222508","DP2OD022552","R01GM1341","S10 OD012372","U01 CA227550","P01 GM121203","CA193417","R35 CA242447","S10‐OD012372","U54 CA193417","MR/T040769/1"],"pubmed_authors":["Swift MF","Stashko C","Viasnoff V","Huang HH","Northey JJ","Volkmann N","Guo W","Ou G","Long AF","Han Y","Bryant DM","Leidal AM","Guo M","Weaver VM","Dumont S","Kai F","Wiita AP","Radhakrishnan R","Tourdot RW","Gaietta G","Hanein D"],"additional_accession":[]},"is_claimable":false,"name":"ECM dimensionality tunes actin tension to modulate endoplasmic reticulum function and spheroid phenotypes of mammary epithelial cells.","description":"Patient-derived organoids and cellular spheroids recapitulate tissue physiology with remarkable fidelity. We investigated how engagement with a reconstituted basement membrane in three dimensions (3D) supports the polarized, stress resilient tissue phenotype of mammary epithelial spheroids. Cells interacting with reconstituted basement membrane in 3D had reduced levels of total and actin-associated filamin and decreased cortical actin tension that increased plasma membrane protrusions to promote negative plasma membrane curvature and plasma membrane protein associations linked to protein secretion. By contrast, cells engaging a reconstituted basement membrane in 2D had high cortical actin tension that forced filamin unfolding and endoplasmic reticulum (ER) associations. Enhanced filamin-ER","dates":{"release":"2022-01-01T00:00:00Z","publication":"2022 Sep","modification":"2026-05-28T01:56:19.591Z","creation":"2025-04-04T20:28:41.023Z"},"accession":"S-EPMC9434103","cross_references":{"pubmed":["35880301"],"doi":["10.15252/embj.2021109205"]}}