biostudies-literatureBeacham GMNational Science Foundation graduate research fellowshipNational Institutes of HealthNIGMS NIH HHSNIH HHSiyac102https://www.ebi.ac.uk/biostudies/studies/S-EPMC9434228biostudies-literatureUnknown222(1)How serine/threonine phosphatases are spatially and temporally tuned by regulatory subunits is a fundamental question in cell biology. Ankyrin repeat, SH3 domain, proline-rich-region-containing proteins are protein phosphatase 1 catalytic subunit binding partners associated with cardiocutaneous diseases. Ankyrin repeat, SH3 domain, proline-rich-region-containing proteins localize protein phosphatase 1 catalytic subunit to cell-cell junctions, but how ankyrin repeat, SH3 domain, proline-rich-region-containing proteins localize and whether they regulate protein phosphatase 1 catalytic subunit activity in vivo is unclear. Through a Caenorhabditis elegans genetic screen, we find that loss of the ankyrin repeat, SH3 domain, proline-rich-region-containing protein homolog, APE-1, suppresses a patGeneticsThe Caenorhabditis elegans ASPP homolog APE-1 is a junctional protein phosphatase 1 modulator.PMC9434228R01 GM129325R01 GM127548DGE-1650441S10 OD018516Wei DTZheng JCamacho MMKZhang YFlorens LHollopeter GBeyrent EBeacham GMfalseThe Caenorhabditis elegans ASPP homolog APE-1 is a junctional protein phosphatase 1 modulator.How serine/threonine phosphatases are spatially and temporally tuned by regulatory subunits is a fundamental question in cell biology. Ankyrin repeat, SH3 domain, proline-rich-region-containing proteins are protein phosphatase 1 catalytic subunit binding partners associated with cardiocutaneous diseases. Ankyrin repeat, SH3 domain, proline-rich-region-containing proteins localize protein phosphatase 1 catalytic subunit to cell-cell junctions, but how ankyrin repeat, SH3 domain, proline-rich-region-containing proteins localize and whether they regulate protein phosphatase 1 catalytic subunit activity in vivo is unclear. Through a Caenorhabditis elegans genetic screen, we find that loss of the ankyrin repeat, SH3 domain, proline-rich-region-containing protein homolog, APE-1, suppresses a pat2022-01-01T00:00:00Z2022 Aug2026-07-14T18:53:36.903Z2024-11-13T10:24:18.353ZS-EPMC94342283579285210.1093/genetics/iyac102