biostudies-literatureXiao CNatural Science Foundation of Hubei ProvinceNatural Science Foundation of Hubei Academy of Agricultural SciencesNational Natural Science Foundation of ChinaNational Key Research and Development Program of China420https://www.ebi.ac.uk/biostudies/studies/S-EPMC9434895biostudies-literatureUnknown22(1)<h4>Background</h4>Cold is a major abiotic stress and Huanglongbing and citrus canker disease are two devastating bacterial diseases for citrus. The Ca²⁺-CBL-CIPK network is known to regulate different types of stress signalling in plants. How do CBL-CIPK signalling networks function in response to cold and infection by CLas or Xcc in citrus?<h4>Results</h4>Eight calcineurin B-like proteins (CBLs) and seventeen CBL-interacting protein kinases (CIPKs) were identified from the cold-tolerant satsuma mandarin 'Guijing2501' (Citrus. unshiu) and CLas/Xcc-sensitive sweet orange (C. sinensis). Phylogenetic analysis revealed that both CBL and CIPK family members in citrus were classified into an ancient and a recent clade according to their conserved domain characteristics and/or intron/BMC plant biologyEvolution, gene expression, and protein‒protein interaction analyses identify candidate CBL-CIPK signalling networks implicated in stress responses to cold and bacterial infection in citrus.PMC94348952020NKYJJ13317019082018YFD10003022019CFB601He XJXie FWang ZQZhang HSun ZHTong ZQiu WMXiao CPan ZYXu YHfalseEvolution, gene expression, and protein‒protein interaction analyses identify candidate CBL-CIPK signalling networks implicated in stress responses to cold and bacterial infection in citrus.<h4>Background</h4>Cold is a major abiotic stress and Huanglongbing and citrus canker disease are two devastating bacterial diseases for citrus. The Ca²⁺-CBL-CIPK network is known to regulate different types of stress signalling in plants. How do CBL-CIPK signalling networks function in response to cold and infection by CLas or Xcc in citrus?<h4>Results</h4>Eight calcineurin B-like proteins (CBLs) and seventeen CBL-interacting protein kinases (CIPKs) were identified from the cold-tolerant satsuma mandarin 'Guijing2501' (Citrus. unshiu) and CLas/Xcc-sensitive sweet orange (C. sinensis). Phylogenetic analysis revealed that both CBL and CIPK family members in citrus were classified into an ancient and a recent clade according to their conserved domain characteristics and/or intron/2022-01-01T00:00:00Z2022 Sep2025-04-19T10:39:27.489Z2025-04-19T10:39:27.489ZS-EPMC94348953604535710.1186/s12870-022-03809-0