{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Cho SW"],"funding":["Ministry of Education","Ministry of Science and ICT, South Korea","National Research Foundation of Korea"],"pagination":["357-365"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC9437366"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["26(5)"],"pubmed_abstract":["Simultaneous myofibril and mitochondrial development is crucial for the cardiac differentiation of pluripotent stem cells (PSCs). Specifically, mitochondrial energy metabolism (MEM) development in cardiomyocytes is essential for the beating function. Although previous studies have reported that MEM is correlated with cardiac differentiation, the process and timing of MEM regulation for cardiac differentiation remain poorly understood. Here, we performed transcriptome analysis of cells at specific stages of cardiac differentiation from mouse embryonic stem cells (mESCs) and human induced PSCs (hiPSCs). We selected MEM genes strongly upregulated at cardiac lineage commitment and in a time-dependent manner during cardiac maturation and identified the protein-protein interaction networks. Notably, MEM proteins were found to interact closely with cardiac maturation-related proteins rather than with cardiac lineage commitment-related proteins. Furthermore, MEM proteins were found to primarily interact with cardiac muscle contractile proteins rather than with cardiac transcription factors. We identified several candidate MEM regulatory genes involved in cardiac lineage commitment (<i>Cck</i>, <i>Bdnf</i>, <i>Fabp4</i>, <i>Cebpα</i>, and <i>Cdkn2a</i> in mESC-derived cells, and <i>CCK</i> and <i>NOS3</i> in hiPSC-derived cells) and cardiac maturation (<i>Ppargc1α</i>, <i>Pgam2</i>, <i>Cox6a2</i>, and <i>Fabp3</i> in mESC-derived cells, and <i>PGAM2</i> and <i>SLC25A4</i> in hiPSC-derived cells). Therefore, our findings show the importance of MEM in cardiac maturation."],"journal":["The Korean journal of physiology & pharmacology : official journal of the Korean Physiological Society and the Korean Society of Pharmacology"],"pubmed_title":["Mitochondrial energy metabolic transcriptome profiles during cardiac differentiation from mouse and human pluripotent stem cells."],"pmcid":["PMC9437366"],"funding_grant_id":["2017R1D1A3B03034465","2020R1C1C1015104","2020R1A4A1018943"],"pubmed_authors":["Kim HK","Sung JH","Kim Y","Kim JH","Cho SW","Han J"],"additional_accession":[]},"is_claimable":false,"name":"Mitochondrial energy metabolic transcriptome profiles during cardiac differentiation from mouse and human pluripotent stem cells.","description":"Simultaneous myofibril and mitochondrial development is crucial for the cardiac differentiation of pluripotent stem cells (PSCs). Specifically, mitochondrial energy metabolism (MEM) development in cardiomyocytes is essential for the beating function. Although previous studies have reported that MEM is correlated with cardiac differentiation, the process and timing of MEM regulation for cardiac differentiation remain poorly understood. Here, we performed transcriptome analysis of cells at specific stages of cardiac differentiation from mouse embryonic stem cells (mESCs) and human induced PSCs (hiPSCs). We selected MEM genes strongly upregulated at cardiac lineage commitment and in a time-dependent manner during cardiac maturation and identified the protein-protein interaction networks. Notably, MEM proteins were found to interact closely with cardiac maturation-related proteins rather than with cardiac lineage commitment-related proteins. Furthermore, MEM proteins were found to primarily interact with cardiac muscle contractile proteins rather than with cardiac transcription factors. We identified several candidate MEM regulatory genes involved in cardiac lineage commitment (<i>Cck</i>, <i>Bdnf</i>, <i>Fabp4</i>, <i>Cebpα</i>, and <i>Cdkn2a</i> in mESC-derived cells, and <i>CCK</i> and <i>NOS3</i> in hiPSC-derived cells) and cardiac maturation (<i>Ppargc1α</i>, <i>Pgam2</i>, <i>Cox6a2</i>, and <i>Fabp3</i> in mESC-derived cells, and <i>PGAM2</i> and <i>SLC25A4</i> in hiPSC-derived cells). Therefore, our findings show the importance of MEM in cardiac maturation.","dates":{"release":"2022-01-01T00:00:00Z","publication":"2022 Sep","modification":"2026-05-28T03:17:55.061Z","creation":"2025-04-07T08:26:52.247Z"},"accession":"S-EPMC9437366","cross_references":{"pubmed":["36039736"],"doi":["10.4196/kjpp.2022.26.5.357"]}}