{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Wilson TL"],"funding":["NIAID NIH HHS","NCI NIH HHS","NIH","NRSA-NIAID","NIH NCI"],"pagination":["2098-2119"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC9437573"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["12(9)"],"pubmed_abstract":["Current chimeric antigen receptor-modified (CAR) T-cell products are evaluated in bulk, without assessing functional heterogeneity. We therefore generated a comprehensive single-cell gene expression and T-cell receptor (TCR) sequencing data set using pre- and postinfusion CD19-CAR T cells from blood and bone marrow samples of pediatric patients with B-cell acute lymphoblastic leukemia. We identified cytotoxic postinfusion cells with identical TCRs to a subset of preinfusion CAR T cells. These effector precursor cells exhibited a unique transcriptional profile compared with other preinfusion cells, corresponding to an unexpected surface phenotype (TIGIT+, CD62Llo, CD27-). Upon stimulation, these cells showed functional superiority and decreased expression of the exhaustion-associated transc"],"journal":["Cancer discovery"],"pubmed_title":["Common Trajectories of Highly Effective CD19-Specific CAR T Cells Identified by Endogenous T-cell Receptor Lineages."],"pmcid":["PMC9437573"],"funding_grant_id":["P30CA021765","F32 AI157296","P30 CA021765","R01 AI136514","F32AI157296","R01AI136514","U01AI150747","U01 AI150747"],"pubmed_authors":["Metais JY","Mettelman RC","Lockey T","Kim H","Allen EK","Talleur AC","Wilson TL","Gottschalk S","Triplett BM","Minervina AA","Meagher MM","Trivedi S","Chou CH","Riberdy JM","Pogorelyy MV","Crawford JC","Velasquez MP","Olsen SR","Willis C","Thomas PG","Langfitt D","Kottapalli P"],"additional_accession":[]},"is_claimable":false,"name":"Common Trajectories of Highly Effective CD19-Specific CAR T Cells Identified by Endogenous T-cell Receptor Lineages.","description":"Current chimeric antigen receptor-modified (CAR) T-cell products are evaluated in bulk, without assessing functional heterogeneity. We therefore generated a comprehensive single-cell gene expression and T-cell receptor (TCR) sequencing data set using pre- and postinfusion CD19-CAR T cells from blood and bone marrow samples of pediatric patients with B-cell acute lymphoblastic leukemia. We identified cytotoxic postinfusion cells with identical TCRs to a subset of preinfusion CAR T cells. These effector precursor cells exhibited a unique transcriptional profile compared with other preinfusion cells, corresponding to an unexpected surface phenotype (TIGIT+, CD62Llo, CD27-). Upon stimulation, these cells showed functional superiority and decreased expression of the exhaustion-associated transc","dates":{"release":"2022-01-01T00:00:00Z","publication":"2022 Sep","modification":"2026-05-28T07:02:02.827Z","creation":"2025-04-06T09:24:17.025Z"},"accession":"S-EPMC9437573","cross_references":{"pubmed":["35792801"],"doi":["10.1158/2159-8290.CD-21-1508"]}}