{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Michaelis JB"],"funding":["Deutsche Forschungsgemeinschaft"],"pagination":["5164"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC9440083"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["13(1)"],"pubmed_abstract":["Mitophagy is essential to maintain mitochondrial function and prevent diseases. It activates upon mitochondria depolarization, which causes PINK1 stabilization on the mitochondrial outer membrane. Strikingly, a number of conditions, including mitochondrial protein misfolding, can induce mitophagy without a loss in membrane potential. The underlying molecular details remain unclear. Here, we report that a loss of mitochondrial protein import, mediated by the pre-sequence translocase-associated motor complex PAM, is sufficient to induce mitophagy in polarized mitochondria. A genome-wide CRISPR/Cas9 screen for mitophagy inducers identifies components of the PAM complex. Protein import defects are able to induce mitophagy without a need for depolarization. Upon mitochondrial protein misfolding"],"journal":["Nature communications"],"pubmed_title":["Protein import motor complex reacts to mitochondrial misfolding by reducing protein import and activating mitophagy."],"pmcid":["PMC9440083"],"funding_grant_id":["390339347","259130777"],"pubmed_authors":["Bozkurt S","Brunstein ME","Wegner M","Pohl C","Kaulich M","Michaelis JB","Alves L","Munch C"],"additional_accession":[]},"is_claimable":false,"name":"Protein import motor complex reacts to mitochondrial misfolding by reducing protein import and activating mitophagy.","description":"Mitophagy is essential to maintain mitochondrial function and prevent diseases. It activates upon mitochondria depolarization, which causes PINK1 stabilization on the mitochondrial outer membrane. Strikingly, a number of conditions, including mitochondrial protein misfolding, can induce mitophagy without a loss in membrane potential. The underlying molecular details remain unclear. Here, we report that a loss of mitochondrial protein import, mediated by the pre-sequence translocase-associated motor complex PAM, is sufficient to induce mitophagy in polarized mitochondria. A genome-wide CRISPR/Cas9 screen for mitophagy inducers identifies components of the PAM complex. Protein import defects are able to induce mitophagy without a need for depolarization. Upon mitochondrial protein misfolding","dates":{"release":"2022-01-01T00:00:00Z","publication":"2022 Sep","modification":"2025-04-19T10:39:06.99Z","creation":"2025-04-19T10:39:06.99Z"},"accession":"S-EPMC9440083","cross_references":{"pubmed":["36056001"],"doi":["10.1038/s41467-022-32564-x"]}}