biostudies-literaturePena-Espinoza MComisión Nacional de Investigación Científica y TecnológicaDanmarks Frie ForskningsfondFondo Nacional de Desarrollo Científico y Tecnológico43-53https://www.ebi.ac.uk/biostudies/studies/S-EPMC9440258biostudies-literatureUnknown20Chagas disease, caused by the protozoa Trypanosoma cruzi, is a potentially life-threatening parasitic zoonosis infecting 6-7 million people worldwide, mainly in Latin America. Due to the limited numbers of drugs available against this neglected disease and their frequent adverse effects, novel anti-chagasic agents are urgently needed. Cichorium intybus L. (chicory) is a bioactive plant with potent activity against parasitic nematodes, but its effects on protozoans are poorly known and no studies have explored its trypanocidal potential. Here, we investigated the activity of C. intybus against extracellular and intracellular stages of T. cruzi, including the prediction of trypanocidal compounds by metabolomic analyses and bioactivity-based molecular networking. Purified C. intybus extracts International journal for parasitology. Drugs and drug resistanceAnti-protozoal activity and metabolomic analyses of Cichorium intybus L. against Trypanosoma cruzi.PMC94402583170875DFF-6111-00394Simonsen HTPena-Espinoza MThamsborg SMLopez-Munoz RValente AHde Roode MWilliams ARRomero-Uzqueda YfalseAnti-protozoal activity and metabolomic analyses of Cichorium intybus L. against Trypanosoma cruzi.Chagas disease, caused by the protozoa Trypanosoma cruzi, is a potentially life-threatening parasitic zoonosis infecting 6-7 million people worldwide, mainly in Latin America. Due to the limited numbers of drugs available against this neglected disease and their frequent adverse effects, novel anti-chagasic agents are urgently needed. Cichorium intybus L. (chicory) is a bioactive plant with potent activity against parasitic nematodes, but its effects on protozoans are poorly known and no studies have explored its trypanocidal potential. Here, we investigated the activity of C. intybus against extracellular and intracellular stages of T. cruzi, including the prediction of trypanocidal compounds by metabolomic analyses and bioactivity-based molecular networking. Purified C. intybus extracts 2022-01-01T00:00:00Z2022 Aug2025-04-18T20:54:21.2Z2025-04-07T08:59:37.773ZS-EPMC94402583603756210.1016/j.ijpddr.2022.08.002