biostudies-literatureNakayama YNHLBI NIH HHS510-527https://www.ebi.ac.uk/biostudies/studies/S-EPMC9444972biostudies-literatureUnknown131(6)<h4>Background</h4>An ancient family of arrestin-fold proteins, termed alpha-arrestins, may have conserved roles in regulating nutrient transporter trafficking and cellular metabolism as adaptor proteins. One alpha-arrestin, TXNIP (thioredoxin-interacting protein), is known to regulate myocardial glucose uptake. However, the in vivo role of the related alpha-arrestin, ARRDC4 (arrestin domain-containing protein 4), is unknown.<h4>Methods</h4>We first tested whether interaction with GLUTs (glucose transporters) is a conserved function of the mammalian alpha-arrestins. To define the in vivo function of ARRDC4, we generated and characterized a novel <i>Arrdc4</i> knockout (KO) mouse model. We then analyzed the molecular interaction between arrestin domains and the basal GLUT1.<h4>Results</h4>ACirculation researchInteraction of ARRDC4 With GLUT1 Mediates Metabolic Stress in the Ischemic Heart.PMC9444972R01 HL130861R01 HL166620Patwari PMukai NNakayama YKreitzer GYoshioka JfalseInteraction of ARRDC4 With GLUT1 Mediates Metabolic Stress in the Ischemic Heart.<h4>Background</h4>An ancient family of arrestin-fold proteins, termed alpha-arrestins, may have conserved roles in regulating nutrient transporter trafficking and cellular metabolism as adaptor proteins. One alpha-arrestin, TXNIP (thioredoxin-interacting protein), is known to regulate myocardial glucose uptake. However, the in vivo role of the related alpha-arrestin, ARRDC4 (arrestin domain-containing protein 4), is unknown.<h4>Methods</h4>We first tested whether interaction with GLUTs (glucose transporters) is a conserved function of the mammalian alpha-arrestins. To define the in vivo function of ARRDC4, we generated and characterized a novel <i>Arrdc4</i> knockout (KO) mouse model. We then analyzed the molecular interaction between arrestin domains and the basal GLUT1.<h4>Results</h4>A2022-01-01T00:00:00Z2022 Sep2026-05-28T14:15:45.443Z2024-11-20T03:03:38.407ZS-EPMC94449723595050010.1161/CIRCRESAHA.122.32135110.1161/circresaha.122.321351