biostudies-literatureSu JIndustrialization of sex control propagation technology of dairy goats and sheep1351-1359https://www.ebi.ac.uk/biostudies/studies/S-EPMC9449401biostudies-literatureUnknown35(9)<h4>Objective</h4>The objective of this study was to analyse the differentially abundant proteins caused by freeze-thawing of ram sperm and explore candidate proteins of interest for their ability to improve ram sperm cryopreservation outcomes in vitro.<h4>Methods</h4>Sperm were from three mature Dorper. Fresh and frozen sperm proteins were extracted, and the differentially abundant proteins were analysed by mass spectrometry. Among these proteins, lactoferrin (LTF) was selected to be added before cryopreservation. Next, sperm samples were diluted in Tris extender, with the addition of 0, 10, 100, 500, and 1,000 μg/mL of LTF. After thawing, sperm quality was evaluated by motility, plasma membrane integrity, mitochondrial activity and reactive oxygen species (ROS).<h4>Results</h4>CryopreserAnimal bioscienceEffect of lactoferrin on ram sperm motility after cryopreservation.PMC94494012020CG0078Yang YCao GWang CSu JSong YfalseEffect of lactoferrin on ram sperm motility after cryopreservation.<h4>Objective</h4>The objective of this study was to analyse the differentially abundant proteins caused by freeze-thawing of ram sperm and explore candidate proteins of interest for their ability to improve ram sperm cryopreservation outcomes in vitro.<h4>Methods</h4>Sperm were from three mature Dorper. Fresh and frozen sperm proteins were extracted, and the differentially abundant proteins were analysed by mass spectrometry. Among these proteins, lactoferrin (LTF) was selected to be added before cryopreservation. Next, sperm samples were diluted in Tris extender, with the addition of 0, 10, 100, 500, and 1,000 μg/mL of LTF. After thawing, sperm quality was evaluated by motility, plasma membrane integrity, mitochondrial activity and reactive oxygen species (ROS).<h4>Results</h4>Cryopreser2022-01-01T00:00:00Z2022 Sep2025-04-26T13:05:26.694Z2025-02-19T04:37:33.845ZS-EPMC94494013524002010.5713/ab.21.0561