{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["9"],"submitter":["Ferraro G"],"funding":["Regione Campania"],"pubmed_abstract":["Artemetin is a valuable 5-hydroxy-3,6,7,3',4'-pentamethoxyflavone present in many different medicinal plants with very good oral bioavailability and drug-likeness values, owing to numerous bioactivities, such as anti-inflammatory and anti-cancer ones. Here, a multi-disciplinary plan has been settled and applied for identifying the artemetin target(s) to inspect its mechanism of action, based on drug affinity-responsive target stability and targeted limited proteolysis. Both approaches point to the disclosure of filamins A and B as direct artemetin targets in HeLa cell lysates, also giving detailed insights into the ligand/protein-binding sites. Interestingly, also 8-prenyl-artemetin, which is an artemetin more permeable semisynthetic analog, directly interacts with filamins A and B. Both c"],"journal":["Frontiers in molecular biosciences"],"pagination":["964295"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC9452882"],"repository":["biostudies-literature"],"pubmed_title":["Drug affinity-responsive target stability unveils filamins as biological targets for artemetin, an anti-cancer flavonoid."],"pmcid":["PMC9452882"],"pubmed_authors":["Stork B","Morretta E","Monti MC","Minassi A","Salamone S","Belvedere R","Pollastro F","Tosco A","Ferraro G","Petrella A"],"additional_accession":[]},"is_claimable":false,"name":"Drug affinity-responsive target stability unveils filamins as biological targets for artemetin, an anti-cancer flavonoid.","description":"Artemetin is a valuable 5-hydroxy-3,6,7,3',4'-pentamethoxyflavone present in many different medicinal plants with very good oral bioavailability and drug-likeness values, owing to numerous bioactivities, such as anti-inflammatory and anti-cancer ones. Here, a multi-disciplinary plan has been settled and applied for identifying the artemetin target(s) to inspect its mechanism of action, based on drug affinity-responsive target stability and targeted limited proteolysis. Both approaches point to the disclosure of filamins A and B as direct artemetin targets in HeLa cell lysates, also giving detailed insights into the ligand/protein-binding sites. Interestingly, also 8-prenyl-artemetin, which is an artemetin more permeable semisynthetic analog, directly interacts with filamins A and B. Both c","dates":{"release":"2022-01-01T00:00:00Z","publication":"2022","modification":"2026-04-08T12:00:18.752Z","creation":"2025-02-19T04:38:33.327Z"},"accession":"S-EPMC9452882","cross_references":{"pubmed":["36090055"],"doi":["10.3389/fmolb.2022.964295"]}}