biostudies-literatureUnknown12Chen Z<i>Phytophthora cinnamomi</i> causes crown and root wilting in more than 5,000 plant species and represents a significant threat to the health of natural ecosystems and horticultural crops. The early and accurate detection of <i>P. cinnamomi</i> is a fundamental step in disease prevention and appropriate management. In this study, based on public genomic sequence data and bioinformatic analysis of several <i>Phytophthora</i>, <i>Phytopythium</i>, and <i>Pythium</i> species, we have identified a new target gene, Pcinn13739; this allowed us to establish a recombinase polymerase amplification-lateral flow dipstick (RPA-LFD) assay for the detection of <i>P. cinnamomi</i>. <i>Pcinn13739</i>-RPA-LFD assay was highly specific to <i>P. cinnamomi</i>. Test results for 12 isolates of <i>P. cinnamomiFrontiers in cellular and infection microbiology923700https://www.ebi.ac.uk/biostudies/studies/S-EPMC9452884biostudies-literatureRapid detection of <i>Phytophthora cinnamomi</i> based on a new target gene <i>Pcinn13739</i>.PMC9452884Jiao BChen ZHe HDai TZhou JfalseRapid detection of <i>Phytophthora cinnamomi</i> based on a new target gene <i>Pcinn13739</i>.<i>Phytophthora cinnamomi</i> causes crown and root wilting in more than 5,000 plant species and represents a significant threat to the health of natural ecosystems and horticultural crops. The early and accurate detection of <i>P. cinnamomi</i> is a fundamental step in disease prevention and appropriate management. In this study, based on public genomic sequence data and bioinformatic analysis of several <i>Phytophthora</i>, <i>Phytopythium</i>, and <i>Pythium</i> species, we have identified a new target gene, Pcinn13739; this allowed us to establish a recombinase polymerase amplification-lateral flow dipstick (RPA-LFD) assay for the detection of <i>P. cinnamomi</i>. <i>Pcinn13739</i>-RPA-LFD assay was highly specific to <i>P. cinnamomi</i>. Test results for 12 isolates of <i>P. cinnamomi2022-01-01T00:00:00Z20222025-04-26T13:48:10.619Z2024-11-20T13:09:04.246ZS-EPMC94528843609319010.3389/fcimb.2022.923700