{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Yang L"],"funding":["International Science &amp; Technology Cooperation Plan of Anhui Province","Innovative Research Groups of Anhui Agricultural University","National Natural Science Foundation of China","Yan Meng"],"pagination":["9891"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC9456260"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["23(17)"],"pubmed_abstract":["<i>BmSuc1</i>, a novel animal-type β-fructofuranosidase (β-FFase, EC 3.2.1.26) encoding gene, was cloned and identified for the first time in the silkworm, <i>Bombyx mori</i>. <i>BmSuc1</i> was specifically and highly expressed in the midgut and silk gland of <i>Bombyx mori</i>. Until now, the function of <i>BmSuc1</i> in the silk gland was unclear. In this study, it was found that the expression changes of <i>BmSuc1</i> in the fifth instar silk gland were consistent with the growth rate of the silk gland. Next, with the aid of the CRISPR/Cas9 system, the <i>BmSuc1</i> locus was genetically mutated, and homozygous mutant silkworm strains with truncated β-FFase (BmSUC1) proteins were established. <i>BmSuc1</i> mutant larvae exhibited stunted growth and decreased body weight. Interestingly, "],"journal":["International journal of molecular sciences"],"pubmed_title":["<i>BmSuc1</i> Affects Silk Properties by Acting on Sericin1 in <i>Bombyx mori</i>."],"pmcid":["PMC9456260"],"funding_grant_id":["ANRC2019032","31872423","2022h11020020"],"pubmed_authors":["Shu R","Liang D","Gan Q","Yang L","Jiang S","Meng Y","Zhao Y","Xie R"],"additional_accession":[]},"is_claimable":false,"name":"<i>BmSuc1</i> Affects Silk Properties by Acting on Sericin1 in <i>Bombyx mori</i>.","description":"<i>BmSuc1</i>, a novel animal-type β-fructofuranosidase (β-FFase, EC 3.2.1.26) encoding gene, was cloned and identified for the first time in the silkworm, <i>Bombyx mori</i>. <i>BmSuc1</i> was specifically and highly expressed in the midgut and silk gland of <i>Bombyx mori</i>. Until now, the function of <i>BmSuc1</i> in the silk gland was unclear. In this study, it was found that the expression changes of <i>BmSuc1</i> in the fifth instar silk gland were consistent with the growth rate of the silk gland. Next, with the aid of the CRISPR/Cas9 system, the <i>BmSuc1</i> locus was genetically mutated, and homozygous mutant silkworm strains with truncated β-FFase (BmSUC1) proteins were established. <i>BmSuc1</i> mutant larvae exhibited stunted growth and decreased body weight. Interestingly, ","dates":{"release":"2022-01-01T00:00:00Z","publication":"2022 Aug","modification":"2026-05-08T03:16:42.582Z","creation":"2024-11-08T20:17:14.155Z"},"accession":"S-EPMC9456260","cross_references":{"pubmed":["36077290"],"doi":["10.3390/ijms23179891"]}}