biostudies-literatureYang LInternational Science &amp; Technology Cooperation Plan of Anhui ProvinceInnovative Research Groups of Anhui Agricultural UniversityNational Natural Science Foundation of ChinaYan Meng9891https://www.ebi.ac.uk/biostudies/studies/S-EPMC9456260biostudies-literatureUnknown23(17)<i>BmSuc1</i>, a novel animal-type β-fructofuranosidase (β-FFase, EC 3.2.1.26) encoding gene, was cloned and identified for the first time in the silkworm, <i>Bombyx mori</i>. <i>BmSuc1</i> was specifically and highly expressed in the midgut and silk gland of <i>Bombyx mori</i>. Until now, the function of <i>BmSuc1</i> in the silk gland was unclear. In this study, it was found that the expression changes of <i>BmSuc1</i> in the fifth instar silk gland were consistent with the growth rate of the silk gland. Next, with the aid of the CRISPR/Cas9 system, the <i>BmSuc1</i> locus was genetically mutated, and homozygous mutant silkworm strains with truncated β-FFase (BmSUC1) proteins were established. <i>BmSuc1</i> mutant larvae exhibited stunted growth and decreased body weight. Interestingly, International journal of molecular sciences<i>BmSuc1</i> Affects Silk Properties by Acting on Sericin1 in <i>Bombyx mori</i>.PMC9456260ANRC2019032318724232022h11020020Shu RLiang DGan QYang LJiang SMeng YZhao YXie Rfalse<i>BmSuc1</i> Affects Silk Properties by Acting on Sericin1 in <i>Bombyx mori</i>.<i>BmSuc1</i>, a novel animal-type β-fructofuranosidase (β-FFase, EC 3.2.1.26) encoding gene, was cloned and identified for the first time in the silkworm, <i>Bombyx mori</i>. <i>BmSuc1</i> was specifically and highly expressed in the midgut and silk gland of <i>Bombyx mori</i>. Until now, the function of <i>BmSuc1</i> in the silk gland was unclear. In this study, it was found that the expression changes of <i>BmSuc1</i> in the fifth instar silk gland were consistent with the growth rate of the silk gland. Next, with the aid of the CRISPR/Cas9 system, the <i>BmSuc1</i> locus was genetically mutated, and homozygous mutant silkworm strains with truncated β-FFase (BmSUC1) proteins were established. <i>BmSuc1</i> mutant larvae exhibited stunted growth and decreased body weight. Interestingly, 2022-01-01T00:00:00Z2022 Aug2026-05-08T03:16:42.582Z2024-11-08T20:17:14.155ZS-EPMC94562603607729010.3390/ijms23179891