{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Jia B"],"funding":["the Guangdong Basic and Applied Basic Research Foundation","the China National Key Research and Development Project for Synthetic Biology","the Research and development plan in key areas of Guangdong Province","the Shenzhen Basic Research Projects","the Guangdong Natural Science Foundation","the National Natural Science Foundation of China"],"pagination":["10176"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC9456367"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["23(17)"],"pubmed_abstract":["Microalgal lipids are essential for biofuel and dietary supplement production. Lipid engineering for higher production has been studied for years. However, due to the complexity of lipid metabolism, single-gene engineering gradually encounters bottlenecks. Multiple gene regulation is more beneficial to boosting lipid accumulation and further clarifying the complex regulatory mechanism of lipid biosynthesis in the homeostasis of lipids, carbohydrates, and protein metabolism. Here, three lipid-related genes, DOF, LACS2, and CIS, were co-regulated in <i>Chlamydomonas reinhartii</i> by two circles of transformation to overexpress DOF and knock down LACS2 and CIS simultaneously. With the multiple regulations of these genes, the intracellular lipids and FA content increased by 142% and 52%, resp"],"journal":["International journal of molecular sciences"],"pubmed_title":["Increased Lipids in <i>Chlamydomonas reinhardtii</i> by Multiple Regulations of DOF, LACS2, and CIS1."],"pmcid":["PMC9456367"],"funding_grant_id":["2020A1515010873","31870343","JCYJ20180507182405562","2020A1515010352","2022B1111070005","41876188","2018YFA0902500","31800300"],"pubmed_authors":["Yang X","Lan C","Li X","Li Y","Huang Y","Jia B","Yin J"],"additional_accession":[]},"is_claimable":false,"name":"Increased Lipids in <i>Chlamydomonas reinhardtii</i> by Multiple Regulations of DOF, LACS2, and CIS1.","description":"Microalgal lipids are essential for biofuel and dietary supplement production. Lipid engineering for higher production has been studied for years. However, due to the complexity of lipid metabolism, single-gene engineering gradually encounters bottlenecks. Multiple gene regulation is more beneficial to boosting lipid accumulation and further clarifying the complex regulatory mechanism of lipid biosynthesis in the homeostasis of lipids, carbohydrates, and protein metabolism. Here, three lipid-related genes, DOF, LACS2, and CIS, were co-regulated in <i>Chlamydomonas reinhartii</i> by two circles of transformation to overexpress DOF and knock down LACS2 and CIS simultaneously. With the multiple regulations of these genes, the intracellular lipids and FA content increased by 142% and 52%, resp","dates":{"release":"2022-01-01T00:00:00Z","publication":"2022 Sep","modification":"2025-04-05T13:55:42.779Z","creation":"2024-11-09T10:28:44.216Z"},"accession":"S-EPMC9456367","cross_references":{"pubmed":["36077572"],"doi":["10.3390/ijms231710176"]}}