biostudies-literatureGodoy CAUniversity of ValleColcienciasUniversity of Tolima9933https://www.ebi.ac.uk/biostudies/studies/S-EPMC9456414biostudies-literatureUnknown23(17)Processes involving lipases in obtaining active pharmaceutical ingredients (APIs) are crucial to increase the sustainability of the industry. Despite their lower production cost, microbial lipases are striking for their versatile catalyzing reactions beyond their physiological role. In the context of taking advantage of microbial lipases in reactions for the synthesis of API building blocks, this review focuses on: (i) the structural origins of the catalytic properties of microbial lipases, including the results of techniques such as single particle monitoring (SPT) and the description of its selectivity beyond the Kazlauskas rule as the "Mirror-Image Packing" or the "Key Region(s) rule influencing enantioselectivity" (KRIE); (ii) immobilization methods given the conferred operative advantInternational journal of molecular sciencesMicrobial Lipases and Their Potential in the Production of Pharmaceutical Building Blocks.PMC9456414N° 005-2021-Project code N°: 660121Grant 745-2016-Project 110671250425CI 71314Barbosa OGodoy CAPardo-Tamayo JSfalseMicrobial Lipases and Their Potential in the Production of Pharmaceutical Building Blocks.Processes involving lipases in obtaining active pharmaceutical ingredients (APIs) are crucial to increase the sustainability of the industry. Despite their lower production cost, microbial lipases are striking for their versatile catalyzing reactions beyond their physiological role. In the context of taking advantage of microbial lipases in reactions for the synthesis of API building blocks, this review focuses on: (i) the structural origins of the catalytic properties of microbial lipases, including the results of techniques such as single particle monitoring (SPT) and the description of its selectivity beyond the Kazlauskas rule as the "Mirror-Image Packing" or the "Key Region(s) rule influencing enantioselectivity" (KRIE); (ii) immobilization methods given the conferred operative advant2022-01-01T00:00:00Z2022 Sep2025-05-29T16:11:52.632Z2025-04-04T09:32:19.88ZS-EPMC94564143607733210.3390/ijms23179933