{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Han S"],"funding":["American Heart Association","HHS | NIH | National Institute of General Medical Sciences","NIGMS NIH HHS","HHS | NIH | National Institute of General Medical Sciences (NIGMS)"],"pagination":["e2205420119"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC9457259"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["119(36)"],"pubmed_abstract":["Although human sperm is morphologically mature in the epididymis, it cannot fertilize eggs before capacitation. Cholesterol efflux from the sperm plasma membrane is a key molecular event essential for cytoplasmic alkalinization and hyperactivation, but the underlying mechanism remains unclear. The human voltage-gated proton (hHv1) channel functions as an acid extruder to regulate intracellular pHs of many cell types, including sperm. Aside from voltage and pH, Hv channels are also regulated by distinct ligands, such as Zn<sup>2+</sup> and albumin. In the present work, we identified cholesterol as an inhibitory ligand of the hHv1 channel and further investigated the underlying mechanism using the single-molecule fluorescence resonance energy transfer (smFRET) approach. Our results indicated"],"journal":["Proceedings of the National Academy of Sciences of the United States of America"],"pubmed_title":["Cholesterol inhibits human voltage-gated proton channel hHv1."],"pmcid":["PMC9457259"],"funding_grant_id":["1R15GM137215-01","R15 GM137315","19AIREA34470007"],"pubmed_authors":["Vance J","Wang S","Peng S","Goodson R","Gamel P","Chu XP","Han S"],"additional_accession":[]},"is_claimable":false,"name":"Cholesterol inhibits human voltage-gated proton channel hHv1.","description":"Although human sperm is morphologically mature in the epididymis, it cannot fertilize eggs before capacitation. Cholesterol efflux from the sperm plasma membrane is a key molecular event essential for cytoplasmic alkalinization and hyperactivation, but the underlying mechanism remains unclear. The human voltage-gated proton (hHv1) channel functions as an acid extruder to regulate intracellular pHs of many cell types, including sperm. Aside from voltage and pH, Hv channels are also regulated by distinct ligands, such as Zn<sup>2+</sup> and albumin. In the present work, we identified cholesterol as an inhibitory ligand of the hHv1 channel and further investigated the underlying mechanism using the single-molecule fluorescence resonance energy transfer (smFRET) approach. Our results indicated","dates":{"release":"2022-01-01T00:00:00Z","publication":"2022 Sep","modification":"2025-04-19T07:34:29.272Z","creation":"2025-04-19T07:34:29.272Z"},"accession":"S-EPMC9457259","cross_references":{"pubmed":["36037383"],"doi":["10.1073/pnas.2205420119"]}}