biostudies-literatureRen WTenth Youth Fund of the First Hospital of Jilin UniversityJilin Provincial Department of science and technology5609https://www.ebi.ac.uk/biostudies/studies/S-EPMC9457787biostudies-literatureUnknown27(17)The TDM of voriconazole which exhibits wide inter-individual variability is indispensable for treatment in clinic. In this study, a method that high-performance liquid chromatography tandem mass spectrometry cubed (HPLC-MS³) is first built and validated to quantify voriconazole in human plasma. The system is composed of Shimadzu Exion LC^TM UPLC coupled with a Qtrap 5500 mass spectrometer. The separation of voriconazole is performed on a Poroshell 120 SB-C18 column at a flow rate of 0.8 mL/min remaining 7 min for each sample. The calibration curves are linear in the concentration range of 0.25-20 μg/mL. Intra-day and inter-day accuracies and precisions are within 8.0% at three concentrations, and the recoveries and matrix effect are all within accepted limits. In termsMolecules (Basel, Switzerland)Comparison of LC-MS³ and LC-MRM Method for Quantifying Voriconazole and Its Application in Therapeutic Drug Monitoring of Human Plasma.PMC9457787JDYY10201901920200404167YYZhang GRen WZhai THuang JYin LfalseComparison of LC-MS³ and LC-MRM Method for Quantifying Voriconazole and Its Application in Therapeutic Drug Monitoring of Human Plasma.The TDM of voriconazole which exhibits wide inter-individual variability is indispensable for treatment in clinic. In this study, a method that high-performance liquid chromatography tandem mass spectrometry cubed (HPLC-MS³) is first built and validated to quantify voriconazole in human plasma. The system is composed of Shimadzu Exion LC^TM UPLC coupled with a Qtrap 5500 mass spectrometer. The separation of voriconazole is performed on a Poroshell 120 SB-C18 column at a flow rate of 0.8 mL/min remaining 7 min for each sample. The calibration curves are linear in the concentration range of 0.25-20 μg/mL. Intra-day and inter-day accuracies and precisions are within 8.0% at three concentrations, and the recoveries and matrix effect are all within accepted limits. In terms2022-01-01T00:00:00Z2022 Aug2025-04-18T18:24:34.949Z2025-04-07T06:01:42.933ZS-EPMC94577873608037410.3390/molecules27175609