biostudies-literatureUnknown10Chang QUltrasensitive and selective determination of biomarkers of the bone metabolism in serum is crucial for early screening, timely treatment, and monitoring of the curative effect of osteoporosis, which is a silent disease with serious health threats. Immunoassay with a simple sensing interface and ultrahigh sensitivity is highly desirable. Herein, a simple electrochemical immunosensor is demonstrated based on gold nanoparticles (AuNPs) electrodeposited on chitosan-reduced graphene oxide (CS-G) composite modified electrode, which can achieve sensitive determination of the important biomarker of bone metabolism, bone gamma-carboxyglutamate protein (BGP). To overcome the agglomeration of graphene and introduce a biocompatible matrix with functional amino groups, CS-G is prepared and modified onFrontiers in chemistry940795https://www.ebi.ac.uk/biostudies/studies/S-EPMC9458950biostudies-literatureSimple immunosensor for ultrasensitive electrochemical determination of biomarker of the bone metabolism in human serum.PMC9458950Chang QHuang JHe LXi FfalseSimple immunosensor for ultrasensitive electrochemical determination of biomarker of the bone metabolism in human serum.Ultrasensitive and selective determination of biomarkers of the bone metabolism in serum is crucial for early screening, timely treatment, and monitoring of the curative effect of osteoporosis, which is a silent disease with serious health threats. Immunoassay with a simple sensing interface and ultrahigh sensitivity is highly desirable. Herein, a simple electrochemical immunosensor is demonstrated based on gold nanoparticles (AuNPs) electrodeposited on chitosan-reduced graphene oxide (CS-G) composite modified electrode, which can achieve sensitive determination of the important biomarker of bone metabolism, bone gamma-carboxyglutamate protein (BGP). To overcome the agglomeration of graphene and introduce a biocompatible matrix with functional amino groups, CS-G is prepared and modified on2022-01-01T00:00:00Z20222025-04-05T10:25:44.429Z2025-04-05T10:25:44.429ZS-EPMC94589503609267210.3389/fchem.2022.940795