biostudies-literatureYe BZhejiang Academy of Forestry Project4https://www.ebi.ac.uk/biostudies/studies/S-EPMC9459437biostudies-literatureUnknown22(5)In this study, a novel β-1,3-glucan recognition protein gene (β-GRP) was identified from Melanotus cribricollis, and its potential role in the immune response was investigated. The full length of β-GRP cDNA (Accession Number: MT941530) was 1644 bp, encoding a protein composed of 428 amino acids. The theoretical molecular weight and the isoelectric point were 51.53 kDa and 6.17, respectively. The amino acid sequence of β-GRP from M. cribricollis was closely related to that of. β-GRP-like from Photinus pyralis, and was predicted to contain conserved GH16 domain without glucanase active site. The results of real-time quantitative PCR showed that fungal infection of Metarhizium pingshaense may significantly upregulated the expression level of β-GRP gene. The RNAi suppression of β-GRP gene exprJournal of insect science (Online)Cloning and Functional Characterization of a Novel β-GRP Gene From Melanotus cribricollis.PMC94594372021F1065-2Li HShen JZhang YYe BSong QWu CShu JfalseCloning and Functional Characterization of a Novel β-GRP Gene From Melanotus cribricollis.In this study, a novel β-1,3-glucan recognition protein gene (β-GRP) was identified from Melanotus cribricollis, and its potential role in the immune response was investigated. The full length of β-GRP cDNA (Accession Number: MT941530) was 1644 bp, encoding a protein composed of 428 amino acids. The theoretical molecular weight and the isoelectric point were 51.53 kDa and 6.17, respectively. The amino acid sequence of β-GRP from M. cribricollis was closely related to that of. β-GRP-like from Photinus pyralis, and was predicted to contain conserved GH16 domain without glucanase active site. The results of real-time quantitative PCR showed that fungal infection of Metarhizium pingshaense may significantly upregulated the expression level of β-GRP gene. The RNAi suppression of β-GRP gene expr2022-01-01T00:00:00Z2022 Sep2025-05-29T16:11:26.403Z2025-04-06T01:49:59.57ZS-EPMC94594373608267710.1093/jisesa/ieac051