biostudies-literatureChen JNEI NIH HHS109163https://www.ebi.ac.uk/biostudies/studies/S-EPMC9466005biostudies-literatureUnknown222Understanding the molecular composition of ocular tissues and fluids could inform new approaches to prevalent causes of blindness. Subretinal fluid accumulating between the photoreceptor outer segments and retinal pigment epithelium (RPE) is potentially a rich source of proteins and lipids normally cycling among outer retinal cells and choroid. Herein, intact post-translationally modified proteins (proteoforms) were extracted from subretinal fluids of five patients with rhegmatogenous retinal detachment (RRD), analyzed by tandem mass spectrometry, and compared to published data on these same proteins as synthesized by other organs. Single-nuclei transcriptomic data from non-diseased human retina/RPE were used to identify whether proteins in subretinal fluid were of potential ocular origin.Experimental eye researchTransthyretin proteoforms of intraocular origin in human subretinal fluid.PMC9466005F30 EY033198Fortmann SDChen JCurcio CACao DCrosson JNFeist RMfalseTransthyretin proteoforms of intraocular origin in human subretinal fluid.Understanding the molecular composition of ocular tissues and fluids could inform new approaches to prevalent causes of blindness. Subretinal fluid accumulating between the photoreceptor outer segments and retinal pigment epithelium (RPE) is potentially a rich source of proteins and lipids normally cycling among outer retinal cells and choroid. Herein, intact post-translationally modified proteins (proteoforms) were extracted from subretinal fluids of five patients with rhegmatogenous retinal detachment (RRD), analyzed by tandem mass spectrometry, and compared to published data on these same proteins as synthesized by other organs. Single-nuclei transcriptomic data from non-diseased human retina/RPE were used to identify whether proteins in subretinal fluid were of potential ocular origin.2022-01-01T00:00:00Z2022 Sep2026-05-27T11:50:06.186Z2025-04-04T08:13:41.885ZS-EPMC94660053576011910.1016/j.exer.2022.109163