{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Zhang D"],"funding":["Defense Advanced Research Projects Agency","National Institute of General Medical Sciences","NIGMS NIH HHS"],"pagination":["4487-4495"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC9469474"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["144(10)"],"pubmed_abstract":["Chemical cross-linking enables rapid identification of RNA-protein and RNA-nucleic acid inter- and intramolecular interactions. However, no method exists to site-specifically and covalently cross-link two user-defined sites within an RNA. Here, we develop RNA-CLAMP, which enables site-specific and enzymatic cross-linking (clamping) of two selected guanine residues within an RNA. Intramolecular clamping can disrupt normal RNA function, whereas subsequent photocleavage of the cross-linker restores activity. We used RNA-CLAMP to clamp two stem loops within the single-guide RNA (sgRNA) of the CRISPR-Cas9 gene editing system via a photocleavable cross-linker, completely inhibiting gene editing. Visible light irradiation cleaved the cross-linker and restored gene editing with high spatiotemporal"],"journal":["Journal of the American Chemical Society"],"pubmed_title":["Site-Specific and Enzymatic Cross-Linking of sgRNA Enables Wavelength-Selectable Photoactivated Control of CRISPR Gene Editing."],"pmcid":["PMC9469474"],"funding_grant_id":["R35 GM141939","R01 GM123285","HR0011-18-2-0039"],"pubmed_authors":["Zhang D","Li Z","Liu L","Tota E","Jin S","Piao X","Zhang X","Devaraj NK","Fu XD"],"additional_accession":[]},"is_claimable":false,"name":"Site-Specific and Enzymatic Cross-Linking of sgRNA Enables Wavelength-Selectable Photoactivated Control of CRISPR Gene Editing.","description":"Chemical cross-linking enables rapid identification of RNA-protein and RNA-nucleic acid inter- and intramolecular interactions. However, no method exists to site-specifically and covalently cross-link two user-defined sites within an RNA. Here, we develop RNA-CLAMP, which enables site-specific and enzymatic cross-linking (clamping) of two selected guanine residues within an RNA. Intramolecular clamping can disrupt normal RNA function, whereas subsequent photocleavage of the cross-linker restores activity. We used RNA-CLAMP to clamp two stem loops within the single-guide RNA (sgRNA) of the CRISPR-Cas9 gene editing system via a photocleavable cross-linker, completely inhibiting gene editing. Visible light irradiation cleaved the cross-linker and restored gene editing with high spatiotemporal","dates":{"release":"2022-01-01T00:00:00Z","publication":"2022 Mar","modification":"2025-04-18T20:48:28.764Z","creation":"2025-04-07T08:47:13.828Z"},"accession":"S-EPMC9469474","cross_references":{"pubmed":["35257575"],"doi":["10.1021/jacs.1c12166"]}}