{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Zhang J"],"funding":["China Agriculture Research System","MOST | National Key Research and Development Program of China","National Natural Science Foundation of China"],"pagination":["e0089522"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC9469705"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["88(17)"],"pubmed_abstract":["The high host specificity of phages is a real challenge in the therapy applications of the individual phages. This study aimed to edit the long tail fiber proteins (<i>pb1</i>) of a T5-like phage to obtain the engineered phages with expanded plaquing host range. Two T5-like Salmonella phages with high genome sequence homology but different plaquing host ranges, narrow-host range phage vB STyj5-1 (STyj5-1) and wide-host range phage vB BD13 (BD13), were isolated and characterized. The <i>pb1</i> parts of STyj5-1 were replaced by the corresponding part of BD13 using homologous recombination method to obtain the engineered phages. The alterations of the whole <i>pb1</i> part or the N-terminal amino acids 1-400 of <i>pb1</i> of STyj5-1 could expand their plaquing host ranges (from 20 strains to"],"journal":["Applied and environmental microbiology"],"pubmed_title":["Expansion of the Plaquing Host Range and Improvement of the Absorption Rate of a T5-like Salmonella Phage by Altering the Long Tail Fibers."],"pmcid":["PMC9469705"],"funding_grant_id":["31870166","2017YFC1600703","CARS-47"],"pubmed_authors":["Jing Y","Zhang J","She J","Ning H","Lin H","Wang J","Wang L"],"additional_accession":[]},"is_claimable":false,"name":"Expansion of the Plaquing Host Range and Improvement of the Absorption Rate of a T5-like Salmonella Phage by Altering the Long Tail Fibers.","description":"The high host specificity of phages is a real challenge in the therapy applications of the individual phages. This study aimed to edit the long tail fiber proteins (<i>pb1</i>) of a T5-like phage to obtain the engineered phages with expanded plaquing host range. Two T5-like Salmonella phages with high genome sequence homology but different plaquing host ranges, narrow-host range phage vB STyj5-1 (STyj5-1) and wide-host range phage vB BD13 (BD13), were isolated and characterized. The <i>pb1</i> parts of STyj5-1 were replaced by the corresponding part of BD13 using homologous recombination method to obtain the engineered phages. The alterations of the whole <i>pb1</i> part or the N-terminal amino acids 1-400 of <i>pb1</i> of STyj5-1 could expand their plaquing host ranges (from 20 strains to","dates":{"release":"2022-01-01T00:00:00Z","publication":"2022 Sep","modification":"2026-06-14T07:47:14.453Z","creation":"2025-02-19T03:26:58.263Z"},"accession":"S-EPMC9469705","cross_references":{"pubmed":["35969059"],"doi":["10.1128/aem.00895-22"]}}