biostudies-literatureZhang JChina Agriculture Research SystemMOST | National Key Research and Development Program of ChinaNational Natural Science Foundation of Chinae0089522https://www.ebi.ac.uk/biostudies/studies/S-EPMC9469705biostudies-literatureUnknown88(17)The high host specificity of phages is a real challenge in the therapy applications of the individual phages. This study aimed to edit the long tail fiber proteins (<i>pb1</i>) of a T5-like phage to obtain the engineered phages with expanded plaquing host range. Two T5-like Salmonella phages with high genome sequence homology but different plaquing host ranges, narrow-host range phage vB STyj5-1 (STyj5-1) and wide-host range phage vB BD13 (BD13), were isolated and characterized. The <i>pb1</i> parts of STyj5-1 were replaced by the corresponding part of BD13 using homologous recombination method to obtain the engineered phages. The alterations of the whole <i>pb1</i> part or the N-terminal amino acids 1-400 of <i>pb1</i> of STyj5-1 could expand their plaquing host ranges (from 20 strains toApplied and environmental microbiologyExpansion of the Plaquing Host Range and Improvement of the Absorption Rate of a T5-like Salmonella Phage by Altering the Long Tail Fibers.PMC9469705318701662017YFC1600703CARS-47Jing YZhang JShe JNing HLin HWang JWang LfalseExpansion of the Plaquing Host Range and Improvement of the Absorption Rate of a T5-like Salmonella Phage by Altering the Long Tail Fibers.The high host specificity of phages is a real challenge in the therapy applications of the individual phages. This study aimed to edit the long tail fiber proteins (<i>pb1</i>) of a T5-like phage to obtain the engineered phages with expanded plaquing host range. Two T5-like Salmonella phages with high genome sequence homology but different plaquing host ranges, narrow-host range phage vB STyj5-1 (STyj5-1) and wide-host range phage vB BD13 (BD13), were isolated and characterized. The <i>pb1</i> parts of STyj5-1 were replaced by the corresponding part of BD13 using homologous recombination method to obtain the engineered phages. The alterations of the whole <i>pb1</i> part or the N-terminal amino acids 1-400 of <i>pb1</i> of STyj5-1 could expand their plaquing host ranges (from 20 strains to2022-01-01T00:00:00Z2022 Sep2026-06-14T07:47:14.453Z2025-02-19T03:26:58.263ZS-EPMC94697053596905910.1128/aem.00895-22