biostudies-literatureUnknown2022Sun Y<h4>Objectives</h4>The aim of this study was to investigate the effect of nitric oxide (NO) on orthodontic tooth movement and the regulatory effect on bone formation.<h4>Design</h4>A mouse orthodontic tooth movement model was established to measure the level of releasing NO. Besides, orthodontic tooth movement distance and the bone formation in the tension side of the orthodontic tooth were also analyzed. In vitro, human periodontal ligament stem cells (hPDLSCs) were cultured under tensile force stimulation. The production of NO and the expression level of nitric oxide synthase (NOS) were detected after mechanical stimulation. Furthermore, the downstream cellular signaling pathway regulated by NO was also explored.<h4>Results</h4>The generation of NO steadily increased throughout the orthoStem cells international4775445https://www.ebi.ac.uk/biostudies/studies/S-EPMC9470363biostudies-literatureForce-Induced Nitric Oxide Promotes Osteogenic Activity during Orthodontic Tooth Movement in Mice.PMC9470363Liu YLi SLin FFu JDu JBai YSun YfalseForce-Induced Nitric Oxide Promotes Osteogenic Activity during Orthodontic Tooth Movement in Mice.<h4>Objectives</h4>The aim of this study was to investigate the effect of nitric oxide (NO) on orthodontic tooth movement and the regulatory effect on bone formation.<h4>Design</h4>A mouse orthodontic tooth movement model was established to measure the level of releasing NO. Besides, orthodontic tooth movement distance and the bone formation in the tension side of the orthodontic tooth were also analyzed. In vitro, human periodontal ligament stem cells (hPDLSCs) were cultured under tensile force stimulation. The production of NO and the expression level of nitric oxide synthase (NOS) were detected after mechanical stimulation. Furthermore, the downstream cellular signaling pathway regulated by NO was also explored.<h4>Results</h4>The generation of NO steadily increased throughout the ortho2022-01-01T00:00:00Z20222025-04-19T07:08:34.012Z2025-04-19T07:08:34.012ZS-EPMC94703633611088910.1155/2022/4775445