biostudies-literatureUnknown148(10)Gambichler TRuhr-Universität Bochum<h4>Purpose</h4>To evaluate the protein expression characteristics of genes employed in a recently introduced prognostic gene expression assay for patients with cutaneous melanoma (CM).<h4>Methods</h4>We studied 37 patients with CM and 10 with benign (melanocytic) nevi (BN). Immunohistochemistry of primary tumor tissue was performed for eight proteins: COL6A6, DCD, GBP4, KLHL41, KRT9, PIP, SCGB1D2, SCGB2A2.<h4>Results</h4>The protein expression of most markers investigated was relatively low (e.g., DCD, KRT9, SCGB1D2) and predominantly cytoplasmatic in melanocytes and keratinocytes. COL6A6, GBP4, and KLHL41 expression was significantly enhanced in CM when compared to BN. DCD protein expression was significantly correlated with COL6A6, GBP4, and KLHL41. GBP4 was positively correlated with KJournal of cancer research and clinical oncology2673-2680https://www.ebi.ac.uk/biostudies/studies/S-EPMC9470607biostudies-literatureProtein expression of prognostic genes in primary melanoma and benign nevi.PMC9470607Nick MKafferlein HULang KBruckmuller SSkrygan MGambichler TSusok LSchroder SStockfleth EMeyer TElfering JWagener DBruning TfalseProtein expression of prognostic genes in primary melanoma and benign nevi.<h4>Purpose</h4>To evaluate the protein expression characteristics of genes employed in a recently introduced prognostic gene expression assay for patients with cutaneous melanoma (CM).<h4>Methods</h4>We studied 37 patients with CM and 10 with benign (melanocytic) nevi (BN). Immunohistochemistry of primary tumor tissue was performed for eight proteins: COL6A6, DCD, GBP4, KLHL41, KRT9, PIP, SCGB1D2, SCGB2A2.<h4>Results</h4>The protein expression of most markers investigated was relatively low (e.g., DCD, KRT9, SCGB1D2) and predominantly cytoplasmatic in melanocytes and keratinocytes. COL6A6, GBP4, and KLHL41 expression was significantly enhanced in CM when compared to BN. DCD protein expression was significantly correlated with COL6A6, GBP4, and KLHL41. GBP4 was positively correlated with K2022-01-01T00:00:00Z2022 Oct2025-04-26T22:27:52.432Z2024-11-09T02:15:05.99ZS-EPMC94706073475753710.1007/s00432-021-03779-0