{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Chou CH"],"funding":["NCATS NIH HHS","NIDDK NIH HHS","NIA NIH HHS","NIAID NIH HHS","National Institutes of Health"],"pagination":["e13682"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC9470889"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["21(9)"],"pubmed_abstract":["Seasonal influenza causes mild to severe respiratory infections and significant morbidity, especially in older adults. Transcriptomic analysis in populations across multiple flu seasons has provided insights into the molecular determinants of vaccine response. Still, the metabolic changes that underlie the immune response to influenza vaccination remain poorly characterized. We performed untargeted metabolomics to analyze plasma metabolites in a cohort of younger and older subjects before and after influenza vaccination to identify vaccine-induced molecular signatures. Metabolomic and transcriptomic data were combined to define networks of gene and metabolic signatures indicative of high and low antibody response in these individuals. We observed age-related differences in metabolic baselines and signatures of antibody response to influenza vaccination and the abundance of α-linolenic and linoleic acids, sterol esters, fatty-acylcarnitines, and triacylglycerol metabolism. We identified a metabolomic signature associated with age-dependent vaccine response, finding increased tryptophan and decreased polyunsaturated fatty acids (PUFAs) in young high responders (HRs), while fatty acid synthesis and cholesteryl esters accumulated in older HRs. Integrated metabolomic and transcriptomic analysis shows that depletion of PUFAs, which are building blocks for prostaglandins and other lipid immunomodulators, in young HR subjects at Day 28 is related to a robust immune response to influenza vaccination. Increased glycerophospholipid levels were associated with an inflammatory response in older HRs to flu vaccination. This multi-omics approach uncovered age-related molecular markers associated with influenza vaccine response and provides insight into vaccine-induced metabolic responses that may help guide development of more effective influenza vaccines."],"journal":["Aging cell"],"pubmed_title":["Metabolomic and transcriptomic signatures of influenza vaccine response in healthy young and older adults."],"pmcid":["PMC9470889"],"funding_grant_id":["K24 AG042489","U19 AI089992","U19 GR101641","UL1 TR001863","P30 DK043351"],"pubmed_authors":["Mohanty S","Joshi SR","Kong L","Jeanfavre S","Avila-Pacheco J","Santori FR","Xavier RJ","Pierce K","Bullock K","Devine L","Raddassi K","Chou CH","Clish C","Kang HA","Ueda I","Shaw AC","Meng H"],"additional_accession":[]},"is_claimable":false,"name":"Metabolomic and transcriptomic signatures of influenza vaccine response in healthy young and older adults.","description":"Seasonal influenza causes mild to severe respiratory infections and significant morbidity, especially in older adults. Transcriptomic analysis in populations across multiple flu seasons has provided insights into the molecular determinants of vaccine response. Still, the metabolic changes that underlie the immune response to influenza vaccination remain poorly characterized. We performed untargeted metabolomics to analyze plasma metabolites in a cohort of younger and older subjects before and after influenza vaccination to identify vaccine-induced molecular signatures. Metabolomic and transcriptomic data were combined to define networks of gene and metabolic signatures indicative of high and low antibody response in these individuals. We observed age-related differences in metabolic baselines and signatures of antibody response to influenza vaccination and the abundance of α-linolenic and linoleic acids, sterol esters, fatty-acylcarnitines, and triacylglycerol metabolism. We identified a metabolomic signature associated with age-dependent vaccine response, finding increased tryptophan and decreased polyunsaturated fatty acids (PUFAs) in young high responders (HRs), while fatty acid synthesis and cholesteryl esters accumulated in older HRs. Integrated metabolomic and transcriptomic analysis shows that depletion of PUFAs, which are building blocks for prostaglandins and other lipid immunomodulators, in young HR subjects at Day 28 is related to a robust immune response to influenza vaccination. Increased glycerophospholipid levels were associated with an inflammatory response in older HRs to flu vaccination. This multi-omics approach uncovered age-related molecular markers associated with influenza vaccine response and provides insight into vaccine-induced metabolic responses that may help guide development of more effective influenza vaccines.","dates":{"release":"2022-01-01T00:00:00Z","publication":"2022 Sep","modification":"2024-12-04T01:43:42.825Z","creation":"2024-12-04T01:43:42.825Z"},"accession":"S-EPMC9470889","cross_references":{"pubmed":["35996998"],"doi":["10.1111/acel.13682"]}}