biostudies-literatureUnknown14(1)Zhai J<h4>Objective</h4>He-Wei Granule (HWKL) is a modern product derived from the modified formulation of traditional Chinese medicine Banxia Xiexin Decoction (BXD), which remarkedly enhanced the anti-proliferation activity of cyclophosphamide (CTX) on HepG2 and SGC-7901 cell lines <i>in vitro</i> in our previous research. The aim of the study was to investigate the synergistic effects of HWKL and CTX using a transplanted H22 hepatocellular carcinoma mouse model.<h4>Methods</h4>The CTX-toxic-reducing efficacy of HWKL was evaluated by hematology indexes, organ indexes and marrow DNA detection. To investigate the underlying mechanisms, histopathology test, immunohistochemistry test and TUNEL staining were conducted. The efficacy of HWKL on the micro-vessel density (MVD) in tumor tissue was also eChinese herbal medicines79-89https://www.ebi.ac.uk/biostudies/studies/S-EPMC9476702biostudies-literatureHe-Wei Granule enhances anti-tumor activity of cyclophosphamide by changing tumor microenvironment.PMC9476702Liu ZHan NZhai JWang YSong ZChang HYin JfalseHe-Wei Granule enhances anti-tumor activity of cyclophosphamide by changing tumor microenvironment.<h4>Objective</h4>He-Wei Granule (HWKL) is a modern product derived from the modified formulation of traditional Chinese medicine Banxia Xiexin Decoction (BXD), which remarkedly enhanced the anti-proliferation activity of cyclophosphamide (CTX) on HepG2 and SGC-7901 cell lines <i>in vitro</i> in our previous research. The aim of the study was to investigate the synergistic effects of HWKL and CTX using a transplanted H22 hepatocellular carcinoma mouse model.<h4>Methods</h4>The CTX-toxic-reducing efficacy of HWKL was evaluated by hematology indexes, organ indexes and marrow DNA detection. To investigate the underlying mechanisms, histopathology test, immunohistochemistry test and TUNEL staining were conducted. The efficacy of HWKL on the micro-vessel density (MVD) in tumor tissue was also e2022-01-01T00:00:00Z2022 Jan2025-04-26T13:48:32.337Z2025-02-19T00:48:12.646ZS-EPMC94767023612012110.1016/j.chmed.2021.10.002