biostudies-literatureZhang QUKRI | Biotechnology and Biological Sciences Research CouncilDepartment of Science and Technology, Hubei Province | Hubei Provincial Central Guidance Local Science and Technology Development ProjectMOST | National Key Research and Development Program of ChinaNational Natural Science Foundation of ChinaBiotechnology and Biological Sciences Research Councile0023922https://www.ebi.ac.uk/biostudies/studies/S-EPMC9476948biostudies-literatureUnknown90(9)Nitrate metabolism is an adaptation mechanism used by many bacteria for survival in anaerobic environments. As a by-product of inflammation, nitrate is used by the intestinal bacterial pathogens to enable gut infection. However, the responses of bacterial respiratory pathogens to nitrate are less well understood. Actinobacillus pleuropneumoniae is an important bacterial respiratory pathogen of swine. Previous studies have suggested that adaptation of A. pleuropneumoniae to anaerobiosis is important for infection. In this work, A. pleuropneumoniae growth and pathogenesis in response to the nitrate were investigated. Nitrate significantly promoted A. pleuropneumoniae growth under anaerobic conditions <i>in vitro</i> and lethality in mice. By using <i>narQ</i> and <i>narP</i> deletion mutantsInfection and immunityThe Metabolic Adaptation in Response to Nitrate Is Critical for Actinobacillus pleuropneumoniae Growth and Pathogenicity under the Regulation of NarQ/P.PMC94769482021YFD1800401BB/K020765/131572535BB/S019901/1No.2020ZYYD029Zhou RHan WYan CLangford PRChen XPeng LBei WHuang QLi LZhang QXu JTang HfalseThe Metabolic Adaptation in Response to Nitrate Is Critical for Actinobacillus pleuropneumoniae Growth and Pathogenicity under the Regulation of NarQ/P.Nitrate metabolism is an adaptation mechanism used by many bacteria for survival in anaerobic environments. As a by-product of inflammation, nitrate is used by the intestinal bacterial pathogens to enable gut infection. However, the responses of bacterial respiratory pathogens to nitrate are less well understood. Actinobacillus pleuropneumoniae is an important bacterial respiratory pathogen of swine. Previous studies have suggested that adaptation of A. pleuropneumoniae to anaerobiosis is important for infection. In this work, A. pleuropneumoniae growth and pathogenesis in response to the nitrate were investigated. Nitrate significantly promoted A. pleuropneumoniae growth under anaerobic conditions <i>in vitro</i> and lethality in mice. By using <i>narQ</i> and <i>narP</i> deletion mutants2022-01-01T00:00:00Z2022 Sep2026-06-14T07:48:29.949Z2025-04-05T20:21:58.124ZS-EPMC94769483593885810.1128/iai.00239-22