biostudies-literatureUnknown21Yuan XThe incorporation of vasculature is known to be effective in tissue or organ functional regeneration. However, a vague understanding of the interaction between epidermal appendages and their vascular niches is a foremost obstacle to obtaining sweat gland (SG)-specific vasculature units. Here, we map their precise anatomical connections and report that the interplay between SG cells (SGCs) and the surrounding vascular niche is key for glandular development and homeostasis maintenance. To replicate this interplay <i>in vitro</i>, we used three-dimensional (3D) bioprinting to generate reproducible SGC spheroids from differentiated adipose-derived mesenchymal stem cells (ADSCs). With dermal microvascular endothelial cells (DMECs), sacrificial templates made from poly (ε-caprolactone) (PCL) werBioactive materials340-357https://www.ebi.ac.uk/biostudies/studies/S-EPMC9483744biostudies-literatureReciprocal interaction between vascular niche and sweat gland promotes sweat gland regeneration.PMC9483744Zhang FFu XZhang MKong DEnhejirigalaZhu MHuang SLi ZLiang LYao BYuan XWang YZhang CSong WKong YDuan XZhu SfalseReciprocal interaction between vascular niche and sweat gland promotes sweat gland regeneration.The incorporation of vasculature is known to be effective in tissue or organ functional regeneration. However, a vague understanding of the interaction between epidermal appendages and their vascular niches is a foremost obstacle to obtaining sweat gland (SG)-specific vasculature units. Here, we map their precise anatomical connections and report that the interplay between SG cells (SGCs) and the surrounding vascular niche is key for glandular development and homeostasis maintenance. To replicate this interplay <i>in vitro</i>, we used three-dimensional (3D) bioprinting to generate reproducible SGC spheroids from differentiated adipose-derived mesenchymal stem cells (ADSCs). With dermal microvascular endothelial cells (DMECs), sacrificial templates made from poly (ε-caprolactone) (PCL) wer2023-01-01T00:00:00Z2023 Mar2025-04-21T18:57:50.124Z2025-04-05T17:25:34.669ZS-EPMC94837443618574510.1016/j.bioactmat.2022.08.021