biostudies-literatureUnknown25(10)Serrano Martinez PDutch Cancer SocietyZonMwStem/progenitor cells are required for maintenance of salivary gland (SG) function and serve as untapped reservoirs to create functional cells. Despite recent advancements in the identification of stem/progenitor pools, in the submandibular gland (SMG), a knowledge gap remains. Furthermore, the contribution to adult SMG homeostasis of stem/progenitor cells originating from embryonic development is unclear. Here, we employ an H2B-GFP embryonic and adult pulse-and-chase system to characterize potential SMG stem/progenitor cells (SGSCs) based on quiescence at different stages. Phenotypical profiling of quiescent cells in the SMG revealed that label-retaining cells (LRCs) of embryonic or adult origin co-localized with CK8+ ductal or vimentin + mesenchymal, but not with CK5+ or CK14 + stem/progiScience105047https://www.ebi.ac.uk/biostudies/studies/S-EPMC9485076biostudies-literatureRole of quiescent cells in the homeostatic maintenance of the adult submandibular salivary gland.PMC9485076Maimets MSerrano Martinez PPringle Svan Os Rde Haan GBron RCoppes RPfalseRole of quiescent cells in the homeostatic maintenance of the adult submandibular salivary gland.Stem/progenitor cells are required for maintenance of salivary gland (SG) function and serve as untapped reservoirs to create functional cells. Despite recent advancements in the identification of stem/progenitor pools, in the submandibular gland (SMG), a knowledge gap remains. Furthermore, the contribution to adult SMG homeostasis of stem/progenitor cells originating from embryonic development is unclear. Here, we employ an H2B-GFP embryonic and adult pulse-and-chase system to characterize potential SMG stem/progenitor cells (SGSCs) based on quiescence at different stages. Phenotypical profiling of quiescent cells in the SMG revealed that label-retaining cells (LRCs) of embryonic or adult origin co-localized with CK8+ ductal or vimentin + mesenchymal, but not with CK5+ or CK14 + stem/prog2022-01-01T00:00:00Z2022 Oct2025-04-18T15:47:57.831Z2025-02-19T01:37:55.453ZS-EPMC94850763614795910.1016/j.isci.2022.105047