biostudies-literatureUnknown13Sun R<i>Fragaria pentaphylla</i>, a wild diploid quinquefoliolate species of <i>Fragaria</i>, is native to Southwest China. It has two morphs of red and white fruit color in nature and has characteristics of unique fragrance and resistance, which made it not only a valuable breeding material but also a potential model plant for molecular function researches. Here, we generate a high-quality chromosome-level genome assembly of a <i>F. pentaphylla</i> accession, BAAFS-FP039 employing a combination of PacBio Long-Read Sequencing, Illumina Short-Read Sequencing, and Hi-C Sequencing. The assembled genome contained 256.74 Mb and a contig N50 length of 32.38 Mb, accounting for 99.9% of the estimated genome (256.77 Mb). Based on Hi-C data, seven pseudo-chromosomes of <i>F. pentaphylla</i>-FP039 genome Frontiers in genetics873711https://www.ebi.ac.uk/biostudies/studies/S-EPMC9485601biostudies-literatureChromosome-level genome assembly of <i>Fragaria pentaphylla</i> using PacBio and Hi-C technologies.PMC9485601Chang LLi SZhong CSun RZhang YDong JZhang HGao YWang GWei LSun JfalseChromosome-level genome assembly of <i>Fragaria pentaphylla</i> using PacBio and Hi-C technologies.<i>Fragaria pentaphylla</i>, a wild diploid quinquefoliolate species of <i>Fragaria</i>, is native to Southwest China. It has two morphs of red and white fruit color in nature and has characteristics of unique fragrance and resistance, which made it not only a valuable breeding material but also a potential model plant for molecular function researches. Here, we generate a high-quality chromosome-level genome assembly of a <i>F. pentaphylla</i> accession, BAAFS-FP039 employing a combination of PacBio Long-Read Sequencing, Illumina Short-Read Sequencing, and Hi-C Sequencing. The assembled genome contained 256.74 Mb and a contig N50 length of 32.38 Mb, accounting for 99.9% of the estimated genome (256.77 Mb). Based on Hi-C data, seven pseudo-chromosomes of <i>F. pentaphylla</i>-FP039 genome 2022-01-01T00:00:00Z20222025-04-18T15:47:47.42Z2025-02-19T01:37:58.094ZS-EPMC94856013614751210.3389/fgene.2022.873711