biostudies-literatureUnknown14(5)Pejler GPrevious research has indicated an intimate functional communication between mast cells (MCs) and neutrophils during inflammatory conditions, but the nature of such communication is not fully understood. Activated neutrophils are known to release DNA-containing extracellular traps (neutrophil extracellular traps [NETs]) and, based on the known ability of tryptase to interact with negatively charged polymers, we here hypothesized that tryptase might interact with NET-contained DNA and thereby regulate NET formation. In support of this, we showed that tryptase markedly enhances NET formation in phorbol myristate acetate-activated human neutrophils. Moreover, tryptase was found to bind vividly to the NETs, to cause proteolysis of core histones and to cause a reduction in the levels of citrullJournal of innate immunity433-446https://www.ebi.ac.uk/biostudies/studies/S-EPMC9485958biostudies-literatureMast Cell Tryptase Potentiates Neutrophil Extracellular Trap Formation.PMC9485958Grujic MRabelo Melo FSommerhoff CPAlanazi SAdler JOlsson AKPejler GfalseMast Cell Tryptase Potentiates Neutrophil Extracellular Trap Formation.Previous research has indicated an intimate functional communication between mast cells (MCs) and neutrophils during inflammatory conditions, but the nature of such communication is not fully understood. Activated neutrophils are known to release DNA-containing extracellular traps (neutrophil extracellular traps [NETs]) and, based on the known ability of tryptase to interact with negatively charged polymers, we here hypothesized that tryptase might interact with NET-contained DNA and thereby regulate NET formation. In support of this, we showed that tryptase markedly enhances NET formation in phorbol myristate acetate-activated human neutrophils. Moreover, tryptase was found to bind vividly to the NETs, to cause proteolysis of core histones and to cause a reduction in the levels of citrull2022-01-01T00:00:00Z20222025-04-04T21:34:50.753Z2025-04-04T21:34:50.753ZS-EPMC94859583493701810.1159/000520972