{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Xu X"],"funding":["NIDDK NIH HHS"],"pagination":["102406"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC9486123"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["298(10)"],"pubmed_abstract":["Preproinsulin entry into the endoplasmic reticulum yields proinsulin, and its subsequent delivery to the distal secretory pathway leads to processing, storage, and secretion of mature insulin. Multiple groups have reported that treatment of pancreatic beta cell lines, rodent pancreatic islets, or human islets with proteasome inhibitors leads to diminished proinsulin and insulin protein levels, diminished glucose-stimulated insulin secretion, and changes in beta-cell gene expression that ultimately lead to beta-cell death. However, these studies have mostly examined treatment times far beyond that needed to achieve acute proteasomal inhibition. Here, we report that although proteasomal inhibition immediately downregulates new proinsulin biosynthesis, it nevertheless acutely increases beta-c"],"journal":["The Journal of biological chemistry"],"pubmed_title":["Proteasomal degradation of WT proinsulin in pancreatic beta cells."],"pmcid":["PMC9486123"],"funding_grant_id":["P30 DK020572","R01 DK048280","R01 DK111174"],"pubmed_authors":["Haataja L","Tsai B","Ji S","Qi L","Liu M","Arvan P","Arunagiri A","Xu X","Alam M"],"additional_accession":[]},"is_claimable":false,"name":"Proteasomal degradation of WT proinsulin in pancreatic beta cells.","description":"Preproinsulin entry into the endoplasmic reticulum yields proinsulin, and its subsequent delivery to the distal secretory pathway leads to processing, storage, and secretion of mature insulin. Multiple groups have reported that treatment of pancreatic beta cell lines, rodent pancreatic islets, or human islets with proteasome inhibitors leads to diminished proinsulin and insulin protein levels, diminished glucose-stimulated insulin secretion, and changes in beta-cell gene expression that ultimately lead to beta-cell death. However, these studies have mostly examined treatment times far beyond that needed to achieve acute proteasomal inhibition. Here, we report that although proteasomal inhibition immediately downregulates new proinsulin biosynthesis, it nevertheless acutely increases beta-c","dates":{"release":"2022-01-01T00:00:00Z","publication":"2022 Oct","modification":"2026-03-31T11:25:00.645Z","creation":"2025-04-05T12:10:44.026Z"},"accession":"S-EPMC9486123","cross_references":{"pubmed":["35988641"],"doi":["10.1016/j.jbc.2022.102406"]}}