biostudies-literatureUnknown8Xu FThe de-methylesterification of the pectic polysaccharide homogalacturonan (HG) by pectin methylesterases (PMEs) is a critical step in the control of plant cell expansion and morphogenesis. Plants have large gene families encoding PMEs but also PME inhibitors (PMEIs) with differ in their biochemical properties. The <i>Arabidopsis thaliana PECTIN METHYLESTERASE INHIBITOR 3 (PMEI3)</i> gene is frequently used as a tool to manipulate pectin methylesterase activity in studies assessing its role in the control of morphogenesis. One limitation of these studies is that the exact biochemical activity of this protein has not yet been determined. In this manuscript we produced the protein in <i>Pichia pastoris</i> and characterized its activity <i>in vitro</i>. Like other PMEIs, PMEI3 inhibits PME acCell surface (Amsterdam, Netherlands)100080https://www.ebi.ac.uk/biostudies/studies/S-EPMC9486134biostudies-literatureBiochemical characterization of Pectin Methylesterase Inhibitor 3 from <i>Arabidopsis thaliana</i>.PMC9486134Domon JMGonneau MSenechal FPeaucelle AHofte HXu FHabrylo OFaucher EMartin MLefebvre VPelloux JfalseBiochemical characterization of Pectin Methylesterase Inhibitor 3 from <i>Arabidopsis thaliana</i>.The de-methylesterification of the pectic polysaccharide homogalacturonan (HG) by pectin methylesterases (PMEs) is a critical step in the control of plant cell expansion and morphogenesis. Plants have large gene families encoding PMEs but also PME inhibitors (PMEIs) with differ in their biochemical properties. The <i>Arabidopsis thaliana PECTIN METHYLESTERASE INHIBITOR 3 (PMEI3)</i> gene is frequently used as a tool to manipulate pectin methylesterase activity in studies assessing its role in the control of morphogenesis. One limitation of these studies is that the exact biochemical activity of this protein has not yet been determined. In this manuscript we produced the protein in <i>Pichia pastoris</i> and characterized its activity <i>in vitro</i>. Like other PMEIs, PMEI3 inhibits PME ac2022-01-01T00:00:00Z2022 Dec2025-04-22T10:59:29.531Z2025-04-05T23:50:36.353ZS-EPMC94861343614770010.1016/j.tcsw.2022.100080